Induction of tissue factor activity in endothelial cells and monocytes by a
modified form of albumin present in normal human plasma
KJ Faucette, CJ Parker, T McCluskey, NJ Bernshaw and GM Rodgers
Department of Pediatrics, University of Utah Medical Center, Salt Lake
City.
Molecules that induce tissue factor expression by responsive cells such as
endothelial cells and monocytes may be important in the regulation of
hemostasis and, perhaps, in mediating certain hemostatic disorders. A
constituent of normal human plasma capable of inducing tissue factor
activity in human endothelial cells and monocytes has been isolated and
identified as a derivative of, or modification associated with albumin.
Procoagulant albumin caused a concentration-dependent induction of tissue
factor expression by human endothelial cells, but bovine endothelial cells
were unresponsive. The dose-response curve developed a plateau phase,
indicating that the capacity of endothelial cells to respond to the
stimulus was finite. The maximum response induced by the procoagulant
albumin was similar to that observed for maximally effective concentrations
of endotoxin, interleukin-1, and tumor necrosis factor. Time-course studies
showed that procoagulant albumin produced peak activity in 4 to 6 hours.
Identification of a procoagulant form of albumin in normal human plasma
suggests a potential role for this constituent in regulation of hemostasis.
Volume 79,
Issue 11,
pp. 2888-2895,
06/01/1992
Copyright © 1992 by The American Society of Hematology
