|
|
 Previous Article | Table of Contents | Next Article 
Evidence for in vivo clonal proliferation of unique population of blood
CD4-/CD8- T cells bearing T-cell receptor alpha and beta chains in two
normal men
Y Kusunoki, Y Hirai, S Kyoizumi and M Akiyama
Department of Radiobiology, Radiation Effects Research Foundation,
Hiroshima, Japan.
Rare T lymphocytes bearing CD3 surface antigen and T-cell receptor (TCR)
alpha and beta chains, but lacking both CD4 and CD8 antigens, viz, TCR
alpha beta+CD4-8- cells, appear at a frequency of 0.1% to 2% in peripheral
blood TCR alpha beta+ cells of normal donors. Here we report two unusual
cases, found among 100 healthy individuals studied, who showed an
abnormally elevated frequency of these T cells, ie, 5% to 10% and 14% to
19%. Southern blot analyses of the TCR alpha beta+CD4-8- clones all showed
the identical rearrangement patterns for each individual, demonstrating
that these are derivatives of a single T cell. The same rearrangement
patterns were also observed for the freshly isolated lymphocytes of TCR
alpha beta+CD4-CD8- fraction, which excludes the possible bias in the
processes of in vitro cloning. These TCR alpha beta+CD4-8- T cells were
found to express other mature T-cell markers such as CD2, CD3, and CD5
antigens, as well as natural killer (NK) cell markers (CD11b, CD16, CD56,
and CD57 antigens) for both individuals. Further, although lectin-dependent
or redirected antibody- dependent cell-mediated cytotoxicities were
observed for both freshly sorted lymphocytes of TCR alpha beta+CD4-8-
fraction and in vitro established clones, NK-like activity was not
detected.
Volume 79,
Issue 11,
pp. 2965-2972,
06/01/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Fischer, S. Voelkl, J. Heymann, G. K. Przybylski, K. Mondal, M. Laumer, L. Kunz-Schughart, C. A. Schmidt, R. Andreesen, and A. Mackensen
Isolation and characterization of human antigen-specific TCR{alpha}{beta}+ CD4-CD8- double-negative regulatory T cells
Blood,
April 1, 2005;
105(7):
2828 - 2835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Oikawa, A. Shimada, S. Yamada, Y. Motohashi, Y. Nakagawa, J.-i. Irie, T. Maruyama, and T. Saruta
High Frequency of V{alpha}24+ V{beta}11+ T-Cells Observed in Type 1 Diabetes
Diabetes Care,
October 1, 2002;
25(10):
1818 - 1823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A Anand, G S Dean, K Quereshi, D A Isenberg, and P M Lydyard
Characterization of CD3+CD4-CD8- (double negative) T cells in patients with systemic lupus erythematosus: activation markers
Lupus,
August 1, 2002;
11(8):
493 - 500.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. C. Sirianni, L. Vincenzi, V. Fiorelli, S. Topino, E. Scala, S. Uccini, A. Angeloni, A. Faggioni, D. Cerimele, F. Cottoni, et al.
gamma -Interferon Production in Peripheral Blood Mononuclear Cells and Tumor Infiltrating Lymphocytes From Kaposi's Sarcoma Patients: Correlation With the Presence of Human Herpesvirus-8 in Peripheral Blood Mononuclear Cells and Lesional Macrophages
Blood,
February 1, 1998;
91(3):
968 - 976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
