|
|
 Previous Article | Table of Contents | Next Article 
Interleukin-2-activated natural killer cells can support hematopoiesis in
vitro and promote marrow engraftment in vivo
WJ Murphy, JR Keller, CL Harrison, HA Young and DL Longo
Biological Response Modifiers Program, National Cancer Institute- Frederick
Cancer Research and Development Center, MD 21702-1201.
Purified natural killer (NK) cells were obtained from mice with severe
combined immune deficiency (SCID) to ascertain their effect on
hematopoiesis. When activated and propagated with recombinant human
interleukin-2 (rhIL-2) in vitro, SCID spleen cells maintained a phenotypic
and lytic spectrum consistent with a pure population of activated NK cells.
When added with syngeneic bone marrow cells (BMC) in soft agar, the
activated NK cells could support hematopoietic growth in vitro without the
addition of exogenous hematopoietic growth factors. However, when syngeneic
BMC were added along with cytokines to produce optimal growth conditions,
the addition of NK cells was then inhibitory for hematopoietic colony
formation. Antibodies to interferon- gamma (IFN-gamma) partially reversed
the inhibitory effects. Supernatants from the NK-cell cultures could also
exert these effects on hematopoiesis, although to a lesser extent. Analysis
of the NK cell RNA demonstrated that activated NK cells express genes for
hematopoietic growth factors such as granulocyte-macrophage colony-
stimulating factor (GM-CSF), granulocyte CSF (G-CSF), and IL-1 beta. The NK
cells were also found to express IFN-gamma, transforming growth factor-beta
1 (TGF-beta 1), and tumor necrosis factor-alpha (TNF-alpha) mRNA. Analysis
of the NK-cell supernatants using factor-dependent myeloid progenitor cell
lines showed that the NK cells were producing G- CSF and growth-promoting
activity that could not be attributed to IL-1, IL-3, IL-4, IL-5, IL-6,
GM-CSF, G-CSF, macrophage CSF (M-CSF), or stem cell factor. The transfer of
activated NK cells with BMC into lethally irradiated syngeneic mice
resulted in greater BMC engraftment in the recipients. Thus, these results
using a pure population of activated NK cells indicate that when activated,
these cells can produce a variety of growth factors for hematopoiesis and
exert significant hematopoietic growth-promoting effects in vivo.
Volume 80,
Issue 3,
pp. 670-677,
08/01/1992
Copyright © 1992 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. A. Olson, R. Zeiser, A. Beilhack, J. J. Goldman, and R. S. Negrin
Tissue-Specific Homing and Expansion of Donor NK Cells in Allogeneic Bone Marrow Transplantation
J. Immunol.,
September 1, 2009;
183(5):
3219 - 3228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Gujar and T. I. Michalak
Primary Occult Hepadnavirus Infection Induces Virus-Specific T-Cell and Aberrant Cytokine Responses in the Absence of Antiviral Antibody Reactivity in the Woodchuck Model of Hepatitis B Virus Infection
J. Virol.,
April 15, 2009;
83(8):
3861 - 3876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kannan-Hayashi, K. Okamura, S. Hattori, M. Kuwamura, E. Higuchi, H. Terayama, M. Moriyama, M. Mukamoto, M. Okada, Y. Ohsugi, et al.
Neuritogenic Effects of T Cell-Derived IL-3 on Mouse Splenic Sympathetic Neurons In Vivo
J. Immunol.,
March 15, 2008;
180(6):
4227 - 4234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Barao, A. M. Hanash, W. Hallett, L. A. Welniak, K. Sun, D. Redelman, B. R. Blazar, R. B. Levy, and W. J. Murphy
Suppression of natural killer cell-mediated bone marrow cell rejection by CD4+CD25+ regulatory T cells
PNAS,
April 4, 2006;
103(14):
5460 - 5465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Catalina, L. Milewich, V. Kumar, and M. Bennett
Dietary Dehydroepiandrosterone Inhibits Bone Marrow and Leukemia Cell Transplants: Role of Food Restriction
Experimental Biology and Medicine,
December 1, 2003;
228(11):
1303 - 1320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Raziuddin, D. L. Longo, M. Bennett, R. Winkler-Pickett, J. R. Ortaldo, and W. J. Murphy
Increased bone marrow allograft rejection by depletion of NK cells expressing inhibitory Ly49 NK receptors for donor class I antigens
Blood,
September 26, 2002;
100(8):
3026 - 3033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Taylor, P. M. Chaudhary, J. Klem, V. Kumar, J. D. Schatzle, and M. Bennett
Inhibition of the Death Receptor Pathway by cFLIP Confers Partial Engraftment of MHC Class I-Deficient Stem Cells and Reduces Tumor Clearance in Perforin-Deficient Mice
J. Immunol.,
October 15, 2001;
167(8):
4230 - 4237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.A. Sosman, P. Stiff, S.M. Moss, P. Sorokin, B. Martone, R. Bayer, K. van Besien, S. Devine, W. Stock, D. Peace, et al.
Pilot Trial of Interleukin-2 With Granulocyte Colony-Stimulating Factor for the Mobilization of Progenitor Cells in Advanced Breast Cancer Patients Undergoing High-Dose Chemotherapy: Expansion of Immune Effectors Within the Stem-Cell Graft and Post-Stem-Cell Infusion
J. Clin. Oncol.,
February 1, 2001;
19(3):
634 - 644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Korten, E. Wilk, J. E. Gessner, D. Meyer, and R. E. Schmidt
Altered Donor and Recipient Ly49+ NK Cell Subsets in Allogeneic H-2d -> H-2b and H-2b -> H-2d Bone Marrow Chimeras
J. Immunol.,
December 1, 1999;
163(11):
5896 - 5905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Gardiner, A. O' Meara, and D. J. Reen
Differential Cytotoxicity of Cord Blood and Bone Marrow-Derived Natural Killer Cells
Blood,
January 1, 1998;
91(1):
207 - 213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. J. Grossman and L. Ratner
Cytokine Expression and Tumorigenicity of Large Granular Lymphocytic Leukemia Cells From Mice Transgenic for the tax Gene of Human T-Cell Leukemia Virus Type I
Blood,
July 15, 1997;
90(2):
783 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
