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Heterogeneous immunophenotype of granular lymphocyte expansions:
differential expression of the CD8 alpha and CD8 beta chains [see comments]
D de Totero, PL Tazzari, JP DiSanto, PF di Celle, D Raspadori, R Conte, M Gobbi, GB Ferrara, N Flomenberg and F Lauria
Istituto Nazionale per la Ricerca sul Cancro (IST), Genova, Italy.
In this study, we have evaluated 14 large granular lymphocyte (LGL)
expansions, 11 of which were CD8+. Analysis of the membrane expression of
the alpha and beta chains of the CD8 antigen, using specific monoclonal
antibodies (MoAbs), has shown that LGL expansions with the CD3+, CD4+,
CD8+, CD57+ T-cell receptor (TcR) alpha beta phenotype bear the CD8
alpha/alpha isoform, while the CD3+, CD4-, CD8+, CD57+ TcR alpha beta
samples were positive for both the CD8 alpha and CD8 beta chains. These
data were confirmed also by messenger RNA analysis. One additional case,
with a peculiar phenotype (CD3-, CD2-, CD4-, CD8+, CD57-) and a germline
configuration of the TcR beta and gamma chain genes, expressed only the CD8
alpha chain. After additional phenotypic analysis with a wider panel of
MoAbs, it was found that the beta chain of the interleukin-2 receptor was
constitutively expressed on the majority of the samples tested, and that
most of the monoclonal samples coexpressed CD45RA/R0 antigens. Using MoAbs
directed against the variable regions of the TcR beta chain, we could show
a preferential V beta region restriction in the CD8+ monoclonal cases. This
more extensive characterization of CD8+ LGL expansions has further
documented the marked heterogeneity within this rare condition and allowed
a better phenotypic dissection between the monoclonal and polyclonal cases.
Volume 80,
Issue 7,
pp. 1765-1773,
10/01/1992
Copyright © 1992 by The American Society of Hematology
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