Enhanced hematopoiesis in vivo and in vitro by splenic stromal cells
derived from the mouse with recombinant granulocyte colony-stimulating
factor
N Fukushima, H Nishina, Y Koishihara and H Ohkawa
Fuji-Gotemba Research Laboratories, Chugai Pharmaceutical Co, Ltd,
Shizuoka, Japan.
Splenic stromal cells (CF-1 cells) were established from a mouse
administered recombinant human granulocyte colony-stimulating factor
(rG-CSF) to clarify the mechanism of splenic extramedullary hematopoiesis
induced by the factor. The cells were negative for alkaline phosphatase,
factor VIII-related antigen, mac I, and phagocytosis. They were positive
for acid phosphatase, collagen type I, collagen type III, and fibronectin.
CF-1 cells were not converted to adipocytes in a confluent culture with
10(-6) mol/L hydrocortisone. [35S]rG-CSF bound to CF-1 cells specifically
in the growth phase but not in the resting phase. The CF-1 cells had
greater colony-stimulating activities than the normal splenic stromal
cells. When CF-1 cells were added to bone marrow cells in the spleen
colony-forming cells (CFU-S) assay, the number of colonies in the spleen
increased between 1.4 and 1.8 times the control without these stromal
cells. On the other hand, the normal splenic stromal cells had no effect on
increasing the number of CFU-S colonies. Therefore, these data suggest that
a factor- dependent hematopoietic microenvironment is generated in the
spleen by rG-CSF, and the stromal cells that have the hematopoietic potency
become dominant in splenic extramedullary hematopoiesis induced by rG- CSF.
Volume 80,
Issue 8,
pp. 1914-1922,
10/15/1992
Copyright © 1992 by The American Society of Hematology
