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Differentiation-dependent expression of sialyl stage-specific embryonic
antigen-1 and I-antigens on human lymphoid cells and its implications for
carbohydrate-mediated adhesion to vascular endothelium
K Ohmori, A Takada, T Yoneda, Y Buma, K Hirashima, K Tsuyuoka, A Hasegawa and R Kannagi
Department of Laboratory Medicine, Kyoto University, School of Medicine,
Japan.
Expression of two developmentally regulated carbohydrate antigens, the
sialyl stage-specific embryonic antigen-1 (SSEA-1) and I-antigens, in human
lymphocytes and lymphocytic leukemia cells was investigated using specific
monoclonal antibodies. Sialyl SSEA-1 was expressed only on natural killer
(NK) cells, and was essentially absent on resting mature T and B cells
among normal peripheral lymphocytes. On the other hand, the I-antigen was
strongly expressed on virtually all mature B cells, moderately expressed on
most mature T cells, but not expressed on NK cells in normal donors.
Expression of the two antigens on normal T and B cells was reversible; in
vitro stimulation of normal lymphocytes with concanavalin A (Con A)
resulted in the loss of I-antigen and appearance of sialyl SSEA-1 on CD3+ T
blasts, whereas stimulation with pokeweed mitogen led to loss of I-antigen
expression and appearance of sialyl SSEA-1 antigen on CD19+ B blasts. Among
lymphocytic leukemia cells, sialyl SSEA-1 was detected primarily on
leukemia cells having immature properties such as most common acute
lymphocytic leukemia (cALL) blasts, while the I-antigen was frequently
expressed on malignant cells having relatively mature properties, such as
those found in adult T- cell leukemia or chronic lymphocytic leukemia, and
only occasionally on cALL blasts. Among normal peripheral lymphocytes, the
sialyl SSEA-1+I- antigen- NK cells selectively underwent E-selectin
(ELAM-1, endothelial- leukocyte adhesion molecule-1)-dependent adhesion to
endothelial cells, while the I-antigen+sialyl SSEA-1- mature T and B cells
did not, in line with the recent finding that sialyl SSEA-1 serves as a
specific ligand for E-selectin. Con A blasts, which are sialyl
SSEA-1+I-antigen- , also exhibited significant E-selectin-dependent
adhesion to endothelial cells. These results indicate that expression of
the sialyl SSEA-1 and I-antigens varies alternately depending on the
differentiation/activation status of the lymphocytes, and that this at
least partly regulates the behavior of lymphocytes at the vessel wall.
Volume 81,
Issue 1,
pp. 101-111,
01/01/1993
Copyright © 1993 by The American Society of Hematology
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