|
|
 Previous Article | Table of Contents | Next Article 
A bone marrow stromal cell line is a source and target for platelet-
derived growth factor
SL Abboud
Department of Medicine, University of Texas Health Science Center, San
Antonio 78284.
Platelet-derived growth factor (PDGF) stimulates multipotent and erythroid
progenitors as well as stromal fibroblasts. Any of the three dimeric forms
of PDGF (AA, AB, or BB) could potentially interact with these cells;
however, the precise cellular origin of PDGF production in the bone marrow
microenvironment is not known. In the present study, we found that medium
conditioned by MBA-2, murine bone marrow-derived endothelial cells,
contains PDGF activity that competes for [125I]PDGF binding to human
foreskin fibroblasts and is mitogenic for these fibroblasts. Northern
analysis of poly(A)+ RNA from MBA-2 shows the expression of both PDGF
A-chain and B-chain mRNAs. Because cytokines such as transforming growth
factor-beta (TGF-beta) regulate hematopoiesis and stimulate PDGF in certain
mesenchymal cells, we determined whether TGF-beta influences PDGF secretion
and gene expression in MBA-2. TGF-beta induced PDGF A-chain and B-chain
mRNAs and the release of PDGF activity. Each of the three PDGF isoforms
also stimulated DNA synthesis in MBA-2, but with different potency (BB >
AB > AA). Ligand binding studies showed specific binding of labeled PDGF
BB and, to a lesser extent, PDGF AA isoform, consistent with predominant
expression of the PDGF-beta receptor in MBA-2. These data show that murine
endothelial stromal cells release PDGF activity and respond to PDGF. Local
production of PDGF in the marrow microenvironment may play an important
role in regulating hematopoietic and stromal cell proliferation.
Volume 81,
Issue 10,
pp. 2547-2553,
05/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Khaldoyanidi, L. Sikora, I. Orlovskaya, V. Matrosova, V. Kozlov, and P. Sriramarao
Correlation between nicotine-induced inhibition of hematopoiesis and decreased CD44 expression on bone marrow stromal cells
Blood,
July 15, 2001;
98(2):
303 - 312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sungaran, O. T. Chisholm, B. Markovic, L. M. Khachigian, Y. Tanaka, and B. H. Chong
The role of platelet alpha -granular proteins in the regulation of thrombopoietin messenger RNA expression in human bone marrow stromal cells
Blood,
May 15, 2000;
95(10):
3094 - 3101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Sintich, M. L. G. Lamm, J. A. Sensibar, and C. Lee
Transforming Growth Factor-{beta}1-Induced Proliferation of the Prostate Cancer Cell Line, TSU-Pr1: The Role of Platelet-Derived Growth Factor
Endocrinology,
August 1, 1999;
140(8):
3411 - 3415.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Q.-R. Chen, C. Miyaura, S. Higashi, M. Murakami, I. Kudo, S. Saito, T. Hiraide, Y. Shibasaki, and T. Suda
Activation of Cytosolic Phospholipase A2 by Platelet-derived Growth Factor Is Essential for Cyclooxygenase-2-dependent Prostaglandin E2 Synthesis in Mouse Osteoblasts Cultured with Interleukin-1
J. Biol. Chem.,
February 28, 1997;
272(9):
5952 - 5958.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
