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Long-term engraftment of normal and post-5-fluorouracil murine marrow into
normal nonmyeloablated mice [see comments]
FM Stewart, RB Crittenden, PA Lowry, S Pearson-White and PJ Quesenberry
Department of Medicine, University of Virginia Health Sciences Center,
Charlottesville.
We report the successful long-term engraftment of normal male donor bone
marrow (BM) transfused into noncytoablated female mice, challenging the
assumption that "niches" need to be created for marrow to engraft. We have
used chromosomal banding and Southern blot analysis to identify
transplanted male marrow cells, and shown the long-term stability of the
chimeric marrows. Balb/C, BDF1, or CBA-J female hosts (no irradiation)
received for 5 consecutive days 40 x 10(6) male cells (per day) of the same
strain, and repopulation patterns were observed. Parallel studies were
performed using tibia/femur equivalents of normal marrow or marrow from
Balb/C mice pretreated 6 days previously with 150 mg/kg 5-fluorouracil
(5-FU). Chromosome banding techniques showed that 5% to 46% of marrow cells
were male 3 to 9 months posttransplant with normal donor marrow. Southern
blot analysis, using the pY2 probe, showed continued engraftment at 21 to
25 months posttransplant, ranging from 15% to 42% male engrafted cells in
marrow. Normal donor male marrow engrafted significantly better than
5-FU-pretreated male marrow as shown 1 to 12 months posttransplant in
non-cytoablated female recipients. Percentages of male engrafted cells in
BM ranged from 23% to 78% for recipients of normal donor marrow and from
0.1% to 39% for recipients of 5-FU marrow. Mean engraftment for 6 mice
receiving normal marrow was 38%, whereas that for 6 mice receiving
post-5-FU marrow was 8%, as assayed 1 to 3 months posttransplant. At 10 to
12 months, mean engraftment for the normal donor group was 46%, compared
with 16% for the 5-FU group. The patterns of engraftment with normal and
5-FU marrow were similar for spleen and thymus. These results show that
long-term chimerism can be established after transplantation of normal
donor marrow to normal nonirradiated host mice and indicate that marrow
spaces do not have to be created for successful engraftment. They suggest
that transplanted marrow competes equally with host marrow for marrow
space. Finally, these data show that post-5-FU Balb/C male marrow is
markedly inferior in the repopulation of Balb/C female host marrow, spleen,
and thymus, and suggest that this population of cells may not be the ideal
population for gene transfer studies.
Volume 81,
Issue 10,
pp. 2566-2571,
05/15/1993
Copyright © 1993 by The American Society of Hematology
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|
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|
|
|
|
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[Abstract]
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|
|
|
|
|
|
|
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December 22, 1998;
95(26):
15155 - 15157.
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[PDF]
|
|
|
|
|
|
|
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May 15, 1998;
91(10):
3681 - 3687.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Nilsson, M. S. Dooner, and P. J. Quesenberry
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December 1, 1997;
90(11):
4646 - 4650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. W. Flake and E. D. Zanjani
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JAMA,
September 17, 1997;
278(11):
932 - 937.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. L.W. Kittler, S. O. Peters, R. B. Crittenden, M. E. Debatis, H. S. Ramshaw, F. M. Stewart, and P. J. Quesenberry
Cytokine-Facilitated Transduction Leads to Low-Level Engraftment in Nonablated Hosts
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90(2):
865 - 872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Nilsson, M. S. Dooner, C. Y. Tiarks, H.-U. Weier, and P. J. Quesenberry
Potential and Distribution of Transplanted Hematopoietic Stem Cells in a Nonablated Mouse Model
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June 1, 1997;
89(11):
4013 - 4020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Prockop
Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
Science,
April 4, 1997;
276(5309):
71 - 74.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. van Os, D. Dawes, J. M.K. Mislow, A. Witsell, and P. M. Mauch
Host Conditioning With 5-Fluorouracil and kit-Ligand to Provide for Long-Term Bone Marrow Engraftment
Blood,
April 1, 1997;
89(7):
2376 - 2383.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Scaradavou, L. Isola, P. Rubinstein, Y. Galperin, V. Najfeld, D. Berlin, J. Gordon, and R. S. Weinberg
A Murine Model for Human Cord Blood Transplantation: Near-Term Fetal and Neonatal Peripheral Blood Cells Can Achieve Long-Term Bone Marrow Engraftment in Sublethally Irradiated Adult Recipients
Blood,
February 1, 1997;
89(3):
1089 - 1099.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
