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Infection of Nippostrongylus brasiliensis induces development of
mucosal-type but not connective tissue-type mast cells in genetically mast
cell-deficient Ws/Ws rats
N Arizono, T Kasugai, M Yamada, M Okada, M Morimoto, H Tei, GF Newlands, HR Miller and Y Kitamura
Department of Medical Zoology, Kyoto Prefectural University of Medicine,
Japan.
Ws/Ws rats have a small deletion at the tyrosine kinase domain of the c-
kit gene and are deficient in both mucosal mast cells (MMC) and connective
tissue-type mast cells (CTMC). The role of the c-kit receptor in the
development of MMC and CTMC was investigated by infecting Ws/Ws and control
+/+ rats with Nippostrongylus brasiliensis (NB), which induces
T-cell-dependent mast cell proliferation. Although mast cells did not
develop in the skin of Ws/Ws rats, a significant number of mast cells
developed in the jejunum after NB infection. These mast cells had the MMC
protease phenotype (rat mast cell protease [RMCP] I-/II+) and lacked
heparin because they were not stained with berberine sulfate. Globule
leukocytes were also detected in the mucosal epithelium of these rats.
However, the number of MMC and the serum concentration of RMCP II in
NB-infected Ws/Ws rats were only 13% and 7% of those of NB-infected +/+
rats, respectively. A small number of mast cells also developed in the
lung, liver, and mesenteric lymph nodes of Ws/Ws rats after NB infection.
Although mast cells in these tissues had the MMC phenotype throughout the
observation period, the increased mast cells in the lung and liver of +/+
rats acquired a CTMC-like phenotype and were RMCP I+/II+, berberine
sulfate+, and formalin resistant. These results indicate that the need for
the stimulus through the c-kit receptor appears to be greater in the
development of CTMC in the skin as well as for CTMC-like mast cells in the
lung and liver than for the development of MMC.
Volume 81,
Issue 10,
pp. 2572-2578,
05/15/1993
Copyright © 1993 by The American Society of Hematology
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