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Stem cell factor amplifies newborn and sickle erythropoiesis in liquid
cultures
RS Weinberg, JC Thomson, R Lao, G Chen and BP Alter
Polly Annenberg Levee Hematology Center, Department of Medicine, Mount
Sinai School of Medicine, New York, NY 10029.
A two-phase liquid-culture system was used to substantially amplify and
differentiate erythroblasts, starting with mononuclear cells from the blood
of normal adults, newborn infants, and patients with sickle cell anemia.
After the first 7 days (phase 1), in medium plus fetal bovine serum (FBS)
alone, or in combination with stem cell factor (SCF) or conditioned medium
(CM), the cell number was unchanged, and the cells all looked like
lymphocytes. These cells were then diluted into medium with erythropoietin
(Ep) alone, with Ep and either SCF or CM, or in methylcellulose with the
same factors (phase 2). After 14 days in liquid phase 2 with SCF and Ep,
the cell numbers increased an average of 30-fold in the sickle, 24-fold in
the newborn, and 4-fold in the normal adult cultures; almost all the cells
were erythroblasts and erythrocytes. SCF in phase 1 increased the number of
late progenitors (CFU-E) assayed in methylcellulose, with the largest
number in sickle, followed by newborn cultures and then adult cultures. We
conclude that erythroid progenitor cells survive for at least 7 days
without Ep (but with FBS). Progenitor cells are amplified, particularly
with SCF. Later in culture, SCF with Ep increases the final number of
differentiated erythroid cells. Both the early and the late effects of SCF
are most effective in sickle, followed by newborn cultures and then adult
cultures.
Volume 81,
Issue 10,
pp. 2591-2599,
05/15/1993
Copyright © 1993 by The American Society of Hematology
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