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Human megakaryocytes have a CD4 molecule capable of binding human
immunodeficiency virus-1
YH Kouri, W Borkowsky, M Nardi, S Karpatkin and RS Basch
Department of Medicine, New York University Medical Center, New York 10016.
Most human megakaryocytes (MGKs) express the CD4 antigen on their surface.
Approximately 25% have a CD4 receptor density comparable to that of CD4+ T
cells (Basch et al, Proc Natl Acad Sci USA 87:8085, 1990). In these
studies, we show: (1) the presence of mRNA for CD4 in human MGKs; (2) the
binding of human immunodeficiency virus-1 (HIV-1) to human MGKs; (3) the
inhibition of binding by anti-CD4 (Leu3a) antibody or rCD4; (4) the
infection of a human MGK line, CHRF-288 with HIV-1; and (5) inhibition of
infection with anti-CD4. Human MGKs have mRNA for CD4 as shown by in situ
hybridization with an RNA probe synthesized from a 3-kb cDNA sequence of
plasmid pSP65.T4.8 containing the full-length CD4 sequence. MGKs (23% +/-
17%) bound HIV-1, as determined by anti-gp120 and anti-CD41 staining.
Binding to human MGKs could be inhibited 55% to 75% with anti-CD4 or rCD4,
respectively. Infection of a CD4+ MGK line (CHRF-288) could be accomplished
with HIV- 1, as determined by proviral DNA polymerase chain reaction and
p24 production. Preincubation with anti-CD4 inhibited apparent proviral DNA
infection by 100% and p24 production by 65% to 70%. Thus, human MGKs have a
CD4 receptor capable of binding HIV-1. Using this receptor, HIV- 1 can
infect cells representative of the MGK lineage.
Volume 81,
Issue 10,
pp. 2664-2670,
05/15/1993
Copyright © 1993 by The American Society of Hematology
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