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Adhesion molecule expression on B-cell chronic lymphocytic leukemia cells:
malignant cell phenotypes define distinct disease subsets
G De Rossi, D Zarcone, F Mauro, G Cerruti, C Tenca, A Puccetti, F Mandelli and CE Grossi
Hematology, Human Biopathology Department, University La Sapienza, Rome,
Italy.
Expression of surface adhesion molecules of the Ig superfamily (CD54 and
CD58), of the integrin family (beta 1, beta 2, and beta 3 chains), of the
selectin family (L-selectin), and of the lymphocyte homing receptor (CD44)
was analyzed on B-cell chronic lymphocytic leukemia (B- CLL) cells from 74
patients. The aim of the study was the definition of phenotypically
distinct disease subsets and the correlation of adhesion molecule
phenotypes with clinical parameters. Expression of CD58 on B- CLL cells
defined more advanced disease stages. In comparison with beta
chain-positive cases, patients whose cells did not express beta 1, beta 2,
and beta 3 integrin chains fell into the most favorable prognostic group,
with lower lymphocytosis and the absence of splenomegaly, diffuse bone
marrow infiltration, and therapy requirement. A novel finding was the
expression of beta 3 chains on cells from a minority (12 of 74) of B-CLL
cases. beta 3 chains were always coexpressed with beta 1 and beta 2 chains.
Two-color immunofluorescence analyses of adhesion molecules such as alpha x
beta 2 integrin (LeuM5) and L- selectin (Leu8) showed that these markers
were detectable on variable proportions of leukemic cells, thus confirming
the intraclonal phenotypic heterogeneity of B-CLL. Differences in the
intensity of CD44 expression were also shown among the various B-CLL
clones. Finally, no major variations were shown by comparison of adhesion
molecule phenotypes of leukemic cells simultaneously obtained from blood
and bone marrow, and of CD5+ versus CD5- clones.
Volume 81,
Issue 10,
pp. 2679-2687,
05/15/1993
Copyright © 1993 by The American Society of Hematology
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