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Phosphatidylinositol 3-kinase associates, via its Src homology 2 domains,
with the activated erythropoietin receptor
JE Damen, AL Mui, L Puil, T Pawson and G Krystal
Terry Fox Laboratory, B.C. Cancer Research Centre, Vancouver, Canada.
The erythropoietin receptor (EpR) belongs to a family of hematopoietin
receptors whose members lack tyrosine kinase activity. Nonetheless, within
minutes of binding Ep, a number of cellular proteins become transiently
phosphorylated on tyrosine residues. One of these proteins, as we and
others have shown previously, is the EpR itself. To identify the remaining
protein substrates, we have examined the antiphosphotyrosine
immunoprecipitates of lysates from Ba/F3 cells expressing high levels of
cell surface EpRs. We now present data showing that, in response to Ep, the
85-Kd regulatory subunit of phosphatidylinositol 3-kinase (PI 3-kinase)
becomes immunoprecipitable with antiphosphotyrosine antibodies. This
appears to be due, in large part, to the specific association of PI
3-kinase with the tyrosine- phosphorylated EpR, either directly or through
a 93- or 70-Kd tyrosine- phosphorylated intermediate. The activity of this
EpR associated PI 3- kinase, assessed in anti-EpR immunoprecipitates, is
maximal within 2 minutes of incubation with Ep and returns almost to
baseline levels by 10 minutes. In vitro studies suggest that the
interaction between PI 3- kinase and the activated EpR is mediated by the
N- and C-terminal SH2 domains of p85 and tyrosine-phosphorylated motifs on
the EpR.
Volume 81,
Issue 12,
pp. 3204-3210,
06/15/1993
Copyright © 1993 by The American Society of Hematology
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|
|
|
|
|
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|
|
|
|
|
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[Abstract]
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|
|
|
|
|
|
|
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[Abstract]
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|
|
|
|
|
|
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[Abstract]
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|
|
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|
|
|
|
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October 17, 1997;
272(42):
26173 - 26178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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14334 - 14340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. Hoefsloot, M. P. van Amelsvoort, L. C.A.M. Broeders, D. C. van der Plas, K. van Lom, H. Hoogerbrugge, I. P. Touw, and B. Lowenberg
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|
|
|
|
|
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February 1, 1997;
89(3):
883 - 895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Feger, A. Dubart, C. Lacout, I. Dusanter-Fourt, P. Mayeux, W. Vainchenker, and D. Dumenil
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Blood,
January 15, 1997;
89(2):
435 - 445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Elliott, T. Lorenzini, D. Chang, J. Barzilay, and E. Delorme
Mapping of the Active Site of Recombinant Human Erythropoietin
Blood,
January 15, 1997;
89(2):
493 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liu, A. B. Jefferson, X. Zhang, F. A. Norris, P. W. Majerus, and G. Krystal
A Novel Phosphatidylinositol-3,4,5-trisphosphate 5-Phosphatase Associates with the Interleukin-3 Receptor
J. Biol. Chem.,
November 22, 1996;
271(47):
29729 - 29733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, K. L. Davis, and A. J. Sytkowski
Protein Kinase C-epsilon Is Necessary for Erythropoietin's Up-regulation of c-myc and for Factor-dependent DNA Synthesis. EVIDENCE FOR DISCRETE SIGNALS FOR GROWTH AND DIFFERENTIATION
J. Biol. Chem.,
October 25, 1996;
271(43):
27025 - 27030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Elliott, T. Lorenzini, D. Yanagihara, D. Chang, and G. Elliott
Activation of the Erythropoietin (EPO) Receptor by Bivalent Anti-EPO Receptor Antibodies
J. Biol. Chem.,
October 4, 1996;
271(40):
24691 - 24697.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Ettinger, R. W. Lauener, and V. Duronio
Protein Kinase C delta Specifically Associates with Phosphatidylinositol 3-Kinase Following Cytokine Stimulation
J. Biol. Chem.,
June 14, 1996;
271(24):
14514 - 14518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Tilbrook, T. Bittorf, S. J. Busfield, D. Chappell, and S. P. Klinken
Disrupted Signaling in a Mutant J2E Cell Line That Shows Enhanced Viability, but Does Not Proliferate or Differentiate, with Erythropoietin
J. Biol. Chem.,
February 16, 1996;
271(7):
3453 - 3459.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Damen, R. L. Cutler, H. Jiao, T. Yi, and G. Krystal
Phosphorylation of Tyrosine 503 in the Erythropoietin Receptor (EpR) Is Essential for Binding the P85 Subunit of Phosphatidylinositol (PI) 3-Kinase and for EpR-associated PI 3-Kinase Activity
J. Biol. Chem.,
October 6, 1995;
270(40):
23402 - 23408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Liu, R. L. Cutler, and G. Krystal
Identification and Characterization of an Interleukin-3 Receptor-associated 110-kDa Serine/Threonine Kinase
J. Biol. Chem.,
September 22, 1995;
270(38):
22422 - 22427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tauchi, G.-S. Feng, R. Shen, M. Hoatlin, G. C. Bagby Jr., D. Kabat, L. Lu, and H. E. Broxmeyer
Involvement of SH2-containing Phosphotyrosine Phosphatase Syp in Erythropoietin Receptor Signal Transduction Pathways
J. Biol. Chem.,
March 10, 1995;
270(10):
5631 - 5635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Verdier, P. Walrafen, N. Hubert, S. Chretien, S. Gisselbrecht, C. Lacombe, and P. Mayeux
Proteasomes Regulate the Duration of Erythropoietin Receptor Activation by Controlling Down-regulation of Cell Surface Receptors
J. Biol. Chem.,
June 9, 2000;
275(24):
18375 - 18381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
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