CD4dull+ hematopoietic progenitor cells in murine bone marrow
M Onishi, K Nagayoshi, K Kitamura, H Hirai, F Takaku and H Nakauchi
Third Department of Internal Medicine, University of Tokyo, Japan.
CD4+ cells comprise approximately 3% to 6% of murine bone marrow (BM)
cells. The majority are CD4dull+, but there are two distinct sub
populations: CD4 brightly positive Gr-1- cells (CD4hiGr-1-) and CD4+ Gr- 1+
cells (CD4loGr-1lo). CD4hiGr-1- cells are considered to be mature T cells
by cell surface antigen expression and morphology. CD4loGr-1lo cells, which
comprise approximately 0.6% of the BM cells, express small amount of B220
and Thy1 antigens. Interestingly, colony-forming units (CFU)-spleen and
CFU-C are not enriched in this population. However, when injected into
lethally irradiated mice, CD4loGr-1lo cells were shown to differentiate
into T-cell, B-cell, and myelo-monocyte lineages when assayed 26 weeks
after transplantation. Furthermore, donor-derived CD4loGr-1lo cells were
present in the recipients' BM at least 16 weeks after transplantation.
These observations suggest that murine CD4loGr- 1lo cells in BM have
self-renewal capability and retain the ability to differentiate into at
least three lineages in long-term hematopoiesis.
Volume 81,
Issue 12,
pp. 3217-3225,
06/15/1993
Copyright © 1993 by The American Society of Hematology
