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Enforced expression of Hlx homeobox gene prompts myeloid cell maturation
and altered adherence properties of T cells
JD Allen and JM Adams
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne
Hospital, Victoria, Australia.
Within the hematopoietic compartment, the murine Hlx homeobox gene is
expressed in myeloid cells, most prominently in macrophages and
granulocytes, and in immature B-lymphoid cells but not in erythroid, mast,
or T-lymphoid cells. The level of Hlx mRNA increased with induced
differentiation of the promyelocytic lines WEHI-3B and HL-60. To address
its biologic action more directly, Hlx expression vectors were introduced
into seven mouse hematopoietic cell lines representing several lineages.
Although four lines did not tolerate stable Hlx expression, high-level
expression was achieved in the early myeloid line FDC-P1 and in the
immature T-cell lines Tikaut and WEHI-707. Overexpression of Hlx in FDC-P1
cells downregulated two markers of myeloid immaturity, Thy-1 and CD34, and
also promoted changes in cellular and colony morphology, indicative of
limited differentiation. Ectopic Hlx expression in the T-cell lines induced
changes in cellular and colony morphology and adhesiveness, concomitant
with decreased expression of adhesion molecules ICAM-1 (CD54) and Pgp-1
(CD44) and increased expression of heat-stable antigen. These results
implicate Hlx in the control of myeloid maturation and the regulation of
lymphoid adhesion processes.
Volume 81,
Issue 12,
pp. 3242-3251,
06/15/1993
Copyright © 1993 by The American Society of Hematology
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