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Topographical dissociation of BCL-2 messenger RNA and protein expression in
human lymphoid tissues
CM Chleq-Deschamps, DP LeBrun, P Huie, DP Besnier, RA Warnke, RK Sibley and ML Cleary
Department of Pathology, Stanford University Medical Center, CA 94305.
Immunohistochemistry and in situ hybridization with a synthetic
oligonucleotide probe were used to compare the topographical distribution
of BCL-2 proto-oncogenic protein with that of its messenger RNA (mRNA) in
normal lymphoid tissues, follicular lymphomas, and lymphoma-derived cell
lines. In normal lymph nodes, BCL-2 protein was most abundant in the small
lymphocytes of primary lymphoid follicles and the mantle zones of secondary
follicles, virtually absent within germinal centers, and of variable
abundance in many interfollicular cells. In contrast, the distribution of
BCL-2 mRNA was roughly reciprocal to that of the protein with intense
hybridization signal in germinal centers and almost none in mantle zones.
Discordant BCL-2 RNA and protein levels were also observed in tonsillar
epithelial cells and cortical thymocytes. Concordant and abundant
expression of BCL-2 mRNA and protein was detected in biopsy tissues and
cell lines from t(14;18)-carrying lymphomas. The contrasting distributions
of BCL- 2 protein and RNA in normal lymphoid tissues suggest that
translational and posttranslational control mechanisms play a significant
role in regulating BCL-2 protein levels in germinal center cells,
epithelial cells, and cortical thymocytes. Concordant BCL-2 mRNA and
protein levels in follicular lymphomas suggest that translational control
mechanisms may be disrupted as part of the sequence of genetic changes that
transforms normal lymphoid cells into neoplastic follicular lymphoma cells.
Volume 81,
Issue 2,
pp. 293-298,
01/15/1993
Copyright © 1993 by The American Society of Hematology
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