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Alpha 4 beta 1 and alpha 5 beta 1 are differentially expressed during
myelopoiesis and mediate the adherence of human CD34+ cells to fibronectin
in an activation-dependent way
JM Kerst, JB Sanders, IC Slaper-Cortenbach, MC Doorakkers, B Hooibrink, RH van Oers, AE von dem Borne and CE van der Schoot
Central Laboratory, Red Cross Blood Transfusion Service, Amsterdam, The
Netherlands.
To study the receptors involved in the interaction between extracellular
matrix proteins and hematopoietic progenitor cells, we analyzed the
expression of beta 1 integrins on CD34+ bone marrow cells by means of
immunoflowcytometry. Alpha 4 beta 1 and alpha 5 beta 1 were expressed,
whereas alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 6 beta 1, and
alpha v beta 1 were virtually absent. Furthermore, we assessed the alpha 4
and alpha 5 expression on committed myeloid progenitor cells. These
colony-forming cells were detected in the alpha 4 dull fraction and the
alpha 5 dull fraction. During myeloid differentiation, both in vivo and in
vitro, a differential expression of alpha 4 beta 1 and alpha 5 beta 1 was
observed. alpha 5 beta 1 was found to be lost at the
myelocytic-metamyelocytic stage, before the loss of alpha 4 beta 1, at the
band stage. Functional studies showed no binding of erythroid
progenitor-depleted, CD34+ bone marrow cells to fibronectin. However,
protein kinase C activation strongly induced fibronectin binding (68% of
the cells). Inhibition experiments with specific antibodies and peptides
showed the binding to be mediated by both alpha 4 beta 1 and alpha 5 beta
1. Also, colony-forming cells of granulocytes and macrophages were
demonstrated to adhere to fibronectin in an activation-dependent way.
During granulocyte colony-stimulating factor-induced in vitro maturation,
the activation-dependent fibronectin binding capacity is gradually lost. We
conclude that: (1) CD34+ bone marrow cells express alpha 4 beta 1 and alpha
5 beta 1; (2) the expression of alpha 4 beta 1 and alpha 5 beta 1 is
differentially expressed during myeloid differentiation; and (3) binding of
CD34+ bone marrow cells to fibronectin is activation dependent.
Volume 81,
Issue 2,
pp. 344-351,
01/15/1993
Copyright © 1993 by The American Society of Hematology
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