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Growth factor requirement for survival in cell-cycle dormancy of primitive
murine lymphohematopoietic progenitors
N Katayama, SC Clark and M Ogawa
Department of Medicine, Medical University of South Carolina, Charleston.
We used enriched marrow cells from mice administered three doses of 150
mg/kg 5-fluorouracil (5-FU) 1, 3 and 7 days before they were killed to
study the effects of different growth factors on the survival of primitive,
cell-cycle dormant progenitors in culture. This cell population yielded
substantially fewer colonies in response to single growth factors than
corresponding preparations from day 2 post-5-FU bone marrow samples, and
the majority of progenitors were multipotential in nature. These
observations were consistent with the prediction that multiple cycles of
5-FU treatment would further enrich for primitive cells. With this cell
population, we found that among all the factors tested, interleukin-3
(IL-3) and steel factor (SF) as single factors are the most effective in
supporting survival of dormant primitive progenitors. Interleukin-6 (IL-6),
granulocyte colony- stimulating factor (G-CSF), interleukin-11 (IL-11),
interleukin-4 (IL- 4), interleukin-1 alpha (IL-1 alpha), and tumor necrosis
factor-alpha (TNF-alpha) also supported survival of a few progenitors, but
much less effectively than either IL-3 or SF. The hematopoietic progenitors
that survived for 1 week in liquid culture supplemented with either IL-3 or
SF retained the capability to develop pre-B-cell colonies in secondary
culture. Our results demonstrate that survival of dormant murine
lymphohematopoietic cells in culture is dependent on the presence of
specific growth factors, and that this growth factor requirement can be
satisfied well by SF or IL-3.
Volume 81,
Issue 3,
pp. 610-616,
02/01/1993
Copyright © 1993 by The American Society of Hematology
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