|
|
 Previous Article | Table of Contents | Next Article 
Expression and functional role of tumor necrosis factor receptors on
leukemic cells from patients with type B chronic lymphoproliferative
disorders
L Trentin, R Zambello, C Agostini, F Siviero, F Adami, R Marcolongo, R Raimondi, T Chisesi, G Pizzolo and G Semenzato
Department of Clinical Medicine, Padua University School of Medicine,
Padova, Italy.
Two receptors for tumor necrosis factor (TNF) with different molecular
weight (75-Kd and 55-Kd) and binding affinity have been recently
discovered. To investigate the distribution and the functional role of
these receptors on leukemic B cells from hairy cell leukemia (HCL) and
B-cell chronic lymphocytic leukemia (B-CLL) patients, we evaluated: (1) the
cytofluorimetric pattern of uncultured and cultured leukemic B cells
incubated with utr-1 and htr-9 monoclonal antibodies (MoAbs), which
specifically recognize the 75-Kd and 55-Kd TNF receptors (TNFR),
respectively; (2) the effect of TNF-alpha and TNF-beta on leukemic B cells
in an in vitro proliferation assay; (3) the role of anti-TNFR MoAbs on
TNF-alpha and TNF-beta-driven B-cell growth; and (4) the proliferative
effect of utr-1 and htr-9 MoAbs on in vitro cultured leukemic cells. Our
study shows that the high affinity (75-Kd) but not the low affinity (55-Kd)
TNFR molecules are expressed on freshly isolated leukemic B cells recovered
from HCL and B-CLL patients. The expression of these receptors was neither
upregulated nor downregulated by different stimuli, including TNF-alpha,
TNF-beta, B-cell growth factor, and interleukin-2. TNF-alpha efficiently
triggers the proliferation of HC and, to a lesser extent, the growth of
B-CLL cells. TNF-beta was also able to transduce the proliferative signal
in HCL, but not in B-CLL patients. TNF-alpha- and TNF-beta-driven B-cell
proliferation was inhibited by the preincubation of leukemic B cells with
utr-1 but not htr-9 MoAb. Moreover, anti-75-Kd, but not anti-55-Kd TNFR
MoAb, was able to trigger the proliferation of leukemic B cells, and in
particular of HC. These results show that leukemic B cells from patients
with HCL and B-CLL are equipped with a fully functional high affinity TNFR.
Volume 81,
Issue 3,
pp. 752-758,
02/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P Miljic, B Bonaci-Nikolic, N Colovic, T Terzic, and M Colovic
Antiribosomal-P protein antibodies in a patient with systemic lupus erythematosus and non-Hodgkin's lymphoma: more than coincidental finding?
Lupus,
January 1, 2009;
18(1):
81 - 85.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Trentin, A. Cabrelle, M. Facco, D. Carollo, M. Miorin, A. Tosoni, P. Pizzo, G. Binotto, L. Nicolardi, R. Zambello, et al.
Homeostatic chemokines drive migration of malignant B cells in patients with non-Hodgkin lymphomas
Blood,
July 15, 2004;
104(2):
502 - 508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Hamano, S. Hirose, A. Ida, M. Abe, D. Zhang, S. Kodera, Y. Jiang, J. Shirai, Y. Miura, H. Nishimura, et al.
Susceptibility Alleles for Aberrant B-1 Cell Proliferation Involved in Spontaneously Occurring B-Cell Chronic Lymphocytic Leukemia in a Model of New Zealand White Mice
Blood,
November 15, 1998;
92(10):
3772 - 3779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Warzocha, P. Ribeiro, J. Bienvenu, P. Roy, C. Charlot, D. Rigal, B. Coiffier, and G. Salles
Genetic Polymorphisms in the Tumor Necrosis Factor Locus Influence Non-Hodgkin's Lymphoma Outcome
Blood,
May 15, 1998;
91(10):
3574 - 3581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hortelano and L. Bosca
6-Mercaptopurine Decreases the Bcl-2/Bax Ratio and Induces Apoptosis in Activated Splenic B Lymphocytes
Mol. Pharmacol.,
March 1, 1997;
51(3):
414 - 421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Santee and L. B. Owen-Schaub
Human Tumor Necrosis Factor Receptor p75/80 (CD120b) Gene Structure and Promoter Characterization
J. Biol. Chem.,
August 30, 1996;
271(35):
21151 - 21159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
