Basic fibroblast growth factor counteracts the suppressive effect of
transforming growth factor-beta 1 on human myeloid progenitor cells
JL Gabrilove, G Wong, E Bollenbacher, K White, S Kojima and EL Wilson
Laboratory of Leukocyte Biology, Sloan-Kettering Institute, Memorial
Sloan-Kettering Cancer Center, New York, NY 10021.
We have previously shown that basic fibroblast growth factor (bFGF) is
mitogenic for human bone marrow stromal cells and enhances myelopoiesis in
human long-term bone marrow culture. In the present study, we examined the
mechanism by which bFGF enhances granulopoiesis. We observed that bFGF
significantly abrogated the inhibitory effect of transforming growth
factor-beta 1 (TGF-beta 1) on granulocyte- macrophage colony-stimulating
factor (GM-CSF)-supported progenitor cell growth (P = .009). The partial
reversal of TGF-beta 1-mediated suppression was dependent on the dose of
bFGF used. In addition, we noted that the inclusion of neutralizing
antibody to TGF-beta 1 significantly augmented the clonogenic response to
GM-CSF. We have also shown that 10 ng/mL or 100 ng/mL of bFGF resulted in a
30% to 100% increase in GM-CSF-mediated progenitor cell growth (P = .0001).
These data suggest that bFGF may enhance myelopoiesis by modulating the
inhibitory response to TGF-beta 1.
Volume 81,
Issue 4,
pp. 909-915,
02/15/1993
Copyright © 1993 by The American Society of Hematology
