|
|
 Previous Article | Table of Contents | Next Article 
Human multipotential progenitor cells (CFU-GEMM) have extensive replating
capacity for secondary CFU-GEMM: an effect enhanced by cord blood plasma
CE Carow, G Hangoc and HE Broxmeyer
Department of Microbiology and Immunology, Indiana University School of
Medicine, Indianapolis 46202-5121.
The replating capability of human umbilical cord blood (CB) multipotential
(CFU-GEMM) progenitors was assessed in vitro as an estimate of self-renewal
using erythropoietin (Epo), steel factor (SLF), and either fetal bovine
serum (FBS) or CB plasma. This study found a much higher replating
efficiency for CB CFU-GEMM than previously reported, in terms of the
percentage of colonies that could be replated, the number of secondary
colonies per replated primary colony, and the size of secondary colonies.
Moreover, the majority of secondary colonies were CFU-GEMM-derived.
Although the percentages of bone marrow CFU-GEMM that replate was similar
to that for CB CFU-GEMM and the sizes of secondary bone marrow and CB
CFU-GEMM were also similar, replated CB CFU-GEMM gave rise to far greater
numbers of secondary colonies. No tertiary colonies were observed when
secondary CFU-GEMM were replated. Detection of extensive secondary
replating potential was enhanced by the addition of CB plasma to the
cultures. This activity was not found in either adult blood (PB) plasma,
umbilical cord vein endothelial cell-conditioned medium (ECCM), FBS plus
ECCM, or FBS plus the combination of interleukin-1 (IL-1), IL-3, IL-6,
IL-11, granulocyte colony-stimulating factor, and granulocyte- macrophage
colony-stimulating factor. Whether the CB plasma-enhancing activity for
CFU-GEMM replating capacity is attributable to a novel factor or factors,
or represents effects of other known cytokines, alone or in combination,
remains to be determined. Of particular relevance, these studies suggest
that human CFU-GEMM have some degree of stemness and perhaps should be
classified as a subset of stem cells.
Volume 81,
Issue 4,
pp. 942-949,
02/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. D. Altenburg, H. E. Broxmeyer, Q. Jin, S. Cooper, S. Basu, and G. Alkhatib
A Naturally Occurring Splice Variant of CXCL12/Stromal Cell-Derived Factor 1 Is a Potent Human Immunodeficiency Virus Type 1 Inhibitor with Weak Chemotaxis and Cell Survival Activities
J. Virol.,
August 1, 2007;
81(15):
8140 - 8148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. E. Broxmeyer, E. F. Srour, G. Hangoc, S. Cooper, S. A. Anderson, and D. M. Bodine
High-efficiency recovery of functional hematopoietic progenitor and stem cells from human cord blood cryopreserved for 15 years
PNAS,
January 21, 2003;
100(2):
645 - 650.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Scaradavou, L. Isola, P. Rubinstein, Y. Galperin, V. Najfeld, D. Berlin, J. Gordon, and R. S. Weinberg
A Murine Model for Human Cord Blood Transplantation: Near-Term Fetal and Neonatal Peripheral Blood Cells Can Achieve Long-Term Bone Marrow Engraftment in Sublethally Irradiated Adult Recipients
Blood,
February 1, 1997;
89(3):
1089 - 1099.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
