Monoclonal antibody J11d.2 recognizes cell cycle-dormant, primitive
hematopoietic progenitors of mice
JP Shih and M Ogawa
Department of Medicine, Medical University of South Carolina, Charleston.
It was reported that monoclonal antibody (MoAb) J11d.2 reacts with mature
blood cells of mice but not with their progenitors. We tested in culture
studies whether this antibody could be used for enrichment for primitive
marrow progenitors. The majority of colony-forming cells including
multipotential progenitors in the marrow cells from 5- fluorouracil
(5-FU)-treated mice were J11d.2+, whereas most of the progenitors from
normal mice were J11d.2-. In addition, formation of multilineage colonies
from J11d.2+ in both 5-FU-treated and normal mice was augmented by
interleukin 6. These observations indicated that MoAb J11d.2 recognizes
cell cycle-dormant progenitors. We have recently described a simple method
that provides 800-fold enrichment for the progenitors in post-5-FU marrow
cells using MoAb D7 (anti-Ly-6A/E). When this method was modified to
include sorting with MoAb J11d.2, D7+ J11d.2+ cells were 2,250-fold
enriched for multipotential progenitors. Micromanipulation and culture of
individual D7+ J11d.2+ cells showed that average plating efficiency of the
cell population is approximately 70% and that about 30% of the progenitors
are lymphohematopoietic in nature. These data demonstrate that J11d.2 is a
useful MoAb for the isolation of primitive hematopoietic progenitors of
mice.
Volume 81,
Issue 5,
pp. 1155-1160,
03/01/1993
Copyright © 1993 by The American Society of Hematology
