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Culture of human fetal B-cell precursors on bone marrow stroma maintains
highly proliferative CD20dim cells
I Moreau, V Duvert, J Banchereau and S Saeland
Schering-Plough Laboratory for Immunological Research, Dardilly, France.
Growth of human B-cell precursors (BCP) was achieved by plating fetal CD10+
surface-mu (s mu)- cells in liquid medium onto bone marrow- derived
fibroblastic stromal cell layers deprived of hematopoietic cells.
Proliferation of the fetal BCP was strongly potentiated by the addition of
interleukin-7 (IL-7) to the cultures. Cultures included both a
stroma-adherent and -nonadherent fraction of lymphoid cells, allowing us to
expand the number of input BCP to 13-fold. In the presence of exogenous
IL-7, proliferation was dose-dependent relative to the number of stromal
cells, demonstrating that soluble IL-7 does not act alone to promote
optimal growth. We further showed that the lymphoid cells recovered remain
CD10+ sIg- BCP and that most cells expressed the maturation-associated CD20
antigen when IL-7 was added to the cultures. Whereas both freshly isolated
CD20- and CD20bright BCP proliferated in the presence of stroma, we
observed that high- proliferative capacity CD20dim cells were maintained in
the cultures. Finally, CD20dim sorted cells were shown to subsequently
acquire high levels of CD20 expression in culture, thus demonstrating a
partial maturation sequence. The present culture system thus represents a
useful model for studying the regulatory signals in early human B
lymphopoiesis.
Volume 81,
Issue 5,
pp. 1170-1178,
03/01/1993
Copyright © 1993 by The American Society of Hematology
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