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Effects of immunosuppressants, FK506, deoxyspergualin, and cyclosporine A
on immature human hematopoiesis
A Hirao, Y Kawano and Y Takaue
Department of Pediatrics, University Hospital of Tokushima, Japan.
Effects of the immunosuppressants, FK506, deoxyspergualin (DSG), and
cyclosporine A (CsA) on the growth of human hematopoietic progenitor cells
were tested in the presence of interleukin-3 (IL-3) with purified bone
marrow and blood cells as targets in methylcellulose culture. FK506 had a
significant stimulatory effect on the growth of colony- forming
units/granulocyte-macrophage (CFU-GM) and burst-forming units/erythroid
(BFU-E) from peripheral blood and cord blood cells but not from bone marrow
cells. Neither DSG nor CsA had an effect on any type of target cell.
Liquid-suspension-limiting dilution assay with IL- 3 showed that FK506
directly stimulated the growth of blood progenitors in a dose-dependent
manner with single-hit kinetics. Liquid-suspension preincubation of blood
cells with FK506 before culture in methylcellulose induced a significant
increase in the amount of IL-3- supported growth of CFU-GM and BFU-E,
whereas initial preincubation with IL-3 and subsequent culture with FK506
plus IL-3 exerted its stimulatory effect only on BFU-E. These data suggest
that the stimulation of hematopoietic progenitor cells by FK506 occurs at a
very early stage of maturation and diminishes with further myeloid
development.
Volume 81,
Issue 5,
pp. 1179-1183,
03/01/1993
Copyright © 1993 by The American Society of Hematology
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