|
|
 Previous Article | Table of Contents | Next Article 
Cooperative effects of interleukin-3 (IL-3), IL-5, and granulocyte-
macrophage colony-stimulating factor: a new myeloid cell line inducible to
eosinophils
CC Paul, M Tolbert, S Mahrer, A Singh, MJ Grace and MA Baumann
Research Service, Dayton VA Medical Center, OH 45428.
The cytokines interleukin-3 (IL-3); IL-5, and granulocyte-macrophage
colony-stimulating factor (GM-CSF) are known to contribute to the
proliferation and differentiation of eosinophil progenitors. Recently, it
was determined that the cellular receptors for these three cytokines share
a common beta-chain while having unique alpha-chains. Thus, there is
considerable interest in how these cytokines and their receptors interact
in promoting production of eosinophils. We have established a cell line
(AML14) from a patient with acute myelogenous leukemia that will
consistently exhibit eosinophilic differentiation in suspension in response
to IL-3, IL-5, and GM-CSF. Proliferation with only modest differentiative
effects was observed in response to a single cytokine. Combinations of two
cytokines gave variable results, with GM-CSF + IL-3 and IL-3 + IL-5 causing
more proliferation than a single cytokine but little more differentiation.
The combination of GM-CSF + IL-5 caused marked enhancement of eosinophilic
differentiation with only modest augmentation of proliferation. The
combination of all three cytokines was most effective in stimulating both
proliferation and eosinophilic differentiation (up to 70% of cells) of
AML14 cells. Specific binding of GM-CSF and IL-5 to AML14 cells can be
conveniently studied by flow cytometric methods, and cross-competition of
these two cytokines for their respective receptors was demonstrated. IL-3
was shown to partially compete for IL-5 binding on AML14 cells. Although
specific IL- 3 binding could not be demonstrated by flow cytometry, mRNA
for the alpha-chains of the IL-3, IL-5, and GM-CSF receptors and the
beta-chain common to all three receptors was detected in AML14 cells. The
AML14 cell line may be a useful model for the study of cooperative
interactions of IL-3, IL-5, GM-CSF, and their respective receptors in the
promotion of eosinophil progenitor growth and differentiation.
Volume 81,
Issue 5,
pp. 1193-1199,
03/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Robert, L. Delva, N. Balitrand, S. Nahajevszky, T. Masszi, C. Chomienne, and B. Papp
Apoptosis Induction by Retinoids in Eosinophilic Leukemia Cells: Implication of Retinoic Acid Receptor-{alpha} Signaling in All-Trans-Retinoic Acid Hypersensitivity.
Cancer Res.,
June 15, 2006;
66(12):
6336 - 6344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Qin, B. Camoretti-Mercado, L. Blokh, C. G. Long, F. D. Ko, and K. J. Hamann
Fas Resistance of Leukemic Eosinophils Is Due to Activation of NF-{kappa}B by Fas Ligation
J. Immunol.,
October 1, 2002;
169(7):
3536 - 3544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Ackerman, L. Liu, M. A. Kwatia, M. P. Savage, D. D. Leonidas, G. J. Swaminathan, and K. R. Acharya
Charcot-Leyden Crystal Protein (Galectin-10) Is Not a Dual Function Galectin with Lysophospholipase Activity but Binds a Lysophospholipase Inhibitor in a Novel Structural Fashion
J. Biol. Chem.,
April 19, 2002;
277(17):
14859 - 14868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Zimmermann, B. L. Daugherty, J. M. Stark, and M. E. Rothenberg
Molecular Analysis of CCR-3 Events in Eosinophilic Cells
J. Immunol.,
January 15, 2000;
164(2):
1055 - 1064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Iwama, J. Pan, P. Zhang, W. Reith, B. Mach, D. G. Tenen, and Z. Sun
Dimeric RFX Proteins Contribute to the Activity and Lineage Specificity of the Interleukin-5 Receptor alpha Promoter through Activation and Repression Domains
Mol. Cell. Biol.,
June 1, 1999;
19(6):
3940 - 3950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Sun, D. A. Yergeau, T. Tuypens, J. Tavernier, C. C. Paul, M. A. Baumann, D. G. Tenen, and S. J. Ackerman
Identification and Characterization of a Functional Promoter Region in the Human Eosinophil IL-5 Receptor alpha Subunit Gene
J. Biol. Chem.,
January 20, 1995;
270(3):
1462 - 1471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Du, Y. M. Alsayed, F. Xin, S. J. Ackerman, and L. C. Platanias
Engagement of the CrkL Adapter in Interleukin-5 Signaling in Eosinophils
J. Biol. Chem.,
October 13, 2000;
275(42):
33167 - 33175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
