|
|
 Previous Article | Table of Contents | Next Article 
Dissecting the hematopoietic microenvironment. IX. Further characterization
of murine bone marrow stromal cells
PE Penn, DZ Jiang, RG Fei, E Sitnicka and NS Wolf
Department of Pathology, School of Medicine, University of Washington,
Seattle 98195.
We have previously shown the adherent nontransformed, nonimmortalized
murine bone marrow stromal cell (BMSC) population to consist of phagocytic
macrophage and endothelial-like cells and nonphagocytic fibroblasts. Both
colonial and near confluent growth of each cell type was obtained following
magnetic bead separation, subsequent passaging, and sustained culture with
fetal bovine serum and cytokines. Monoclonal antibody staining of antigenic
determinants was used to characterize the phenotype of the stromal cell
population in primary platings of murine colony-forming unit fibroblast and
long-term bone marrow cultures. The antibodies MECA-99, MECA-32, and
MJ7-18, raised against murine vascular endothelial antigenic determinants,
and von Willebrand's factor all stained selectively for the rounded
endothelial- like cells. Endothelial-like cells as well as macrophages
expressed the myeloid surface antigens F4/80, 7/4, and Mac-1 under our
culture conditions. The cytoskeleton of the stromal fibroblasts in culture
was shown to express smooth muscle-specific actin isoforms, as evidenced by
positive staining of stress fibers for alpha smooth muscle-1, CGA-7
(alpha/gamma isoforms), and HHF-35 (recognizes all muscle-specific actins).
Under culture conditions, stromal fibroblasts were also found to be
positive for a polyclonal smooth muscle myosin. It was found that these
fibroblasts stained for collagens type I, III, and IV in our cultures.
Although collagen type IV is considered a by-product of endothelial cells,
endothelial-like cells in our cultures did not stain for any of the
collagen types. We propose a classification listing for murine BMSCs as
macrophages, endothelial-like cells, and fibroblasts that display smooth
muscle-like characteristics in culture.
Volume 81,
Issue 5,
pp. 1205-1213,
03/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Ogawa, A. C. LaRue, and C. J. Drake
Hematopoietic origin of fibroblasts/myofibroblasts: its pathophysiologic implications
Blood,
November 1, 2006;
108(9):
2893 - 2896.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Anghelina, P. Krishnan, L. Moldovan, and N. I. Moldovan
Monocytes/Macrophages Cooperate with Progenitor Cells during Neovascularization and Tissue Repair: Conversion of Cell Columns into Fibrovascular Bundles
Am. J. Pathol.,
February 1, 2006;
168(2):
529 - 541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Yao, T. Yokota, L. Xia, P. W. Kincade, and R. P. McEver
Bone marrow dysfunction in mice lacking the cytokine receptor gp130 in endothelial cells
Blood,
December 15, 2005;
106(13):
4093 - 4101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Song, C. N. Pagel, T. M. Campbell, R. N. Pike, and E. J. Mackie
The Role of Protease-Activated Receptor-1 in Bone Healing
Am. J. Pathol.,
March 1, 2005;
166(3):
857 - 868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. del Carmen Rodriguez, A. Bernad, and M. Aracil
Interleukin-6 deficiency affects bone marrow stromal precursors, resulting in defective hematopoietic support
Blood,
May 1, 2004;
103(9):
3349 - 3354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Yoder and K. Hiatt
Engraftment of Embryonic Hematopoietic Cells in Conditioned Newborn Recipients
Blood,
March 15, 1997;
89(6):
2176 - 2183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sigounas, M. Steiner, A. Anagnostou, and G. Sigounas
Synergism of Hemopoietic Growth Factors on Endothelial Cell Proliferation
Angiology,
February 1, 1997;
48(2):
141 - 147.
[Abstract]
[PDF]
|
|
|
|
|
