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Synergism of interferon-gamma and stem cell factor on the development of
murine hematopoietic progenitors in serum-free culture
M Shiohara, K Koike and T Nakahata
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto,
Japan.
We examined the effects of interferon-gamma (IFN-gamma) on the growth of
murine hematopoietic progenitors supported by interleukin-3 (IL-3) or stem
cell factor (SCF) in a serum-free culture system. IFN-gamma inhibited
IL-3-dependent granulocyte-macrophage colony growth by normal bone marrow
cells, but increased the number of pure and mixed megakaryocyte colonies by
post-5-fluorouracil bone marrow cells. The addition of IFN-gamma to the
culture containing SCF resulted in a synergistic action on the development
of primitive hematopoietic progenitors as well as on the development of
mature populations. Primitive progenitors responding to SCF + IFN-gamma
were suggested to be supported by SCF in the early stage of development and
require IFN- gamma for subsequent growth. Replating experiments of blast
cell colonies and comparison of total colony growth among SCF + IFN-gamma,
SCF + IL-3, and SCF + IFN-gamma + IL-3 suggest that multipotential
progenitors supported by SCF + IFN-gamma are a part of those reactive to
SCF + IL-3. These findings suggest that IFN-gamma has bifunctional activity
on murine hematopoiesis.
Volume 81,
Issue 6,
pp. 1435-1441,
03/15/1993
Copyright © 1993 by The American Society of Hematology
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