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Granulocyte colony-stimulating factor induces hFc gamma RI (CD64
antigen)-positive neutrophils via an effect on myeloid precursor cells
JM Kerst, JG van de Winkel, AH Evans, M de Haas, IC Slaper-Cortenbach, TP de Wit, AE von dem Borne, CE van der Schoot and RH van Oers
Central Laboratory of the Red Cross Blood Transfusion Service, Amsterdam,
The Netherlands.
In this study we have examined hFc gamma RI expression during myelopoiesis.
Normal bone marrow (BM) cells were found to express hFc gamma RI up to the
metamyelocyte stage. A different Fc gamma RI expression pattern was
observed in an in vitro model of myelopoiesis. Purified CD34-positive BM
cells, cultured for 12 to 14 days with granulocyte colony-stimulating
factor (G-CSF), differentiate into a population of mature granulocytic
cells. In these cultures, in which hFc gamma RI was virtually absent on the
initial CD34-positive BM cells, hFc gamma RI was strongly induced by G-CSF
after only 5 days. During final maturation the cells remained hFc gamma RI
positive. This expression was confirmed functionally by antibody-sensitized
erythrocytes (EA)-rosette assays. Moreover, the mature myeloid cells were
found to express mRNA encoding for hFc gamma RI, whereas reverse-
transcriptase polymerase chain reaction analysis showed that both hFc gamma
RIA and hFc gamma RIB genes were expressed. In contrast, on peripheral
blood (PB) polymorphonuclear neutrophil leukocytes (PMN) the in vitro
effect of G-CSF as to hFc gamma RI induction was limited. Therefore, we
conclude that, with respect to hFc gamma RI expression on PMN, G-CSF acts
on myeloid precursor cells rather than on mature cells. This conclusion
could be strengthened by in vivo administration of a single dose of G-CSF
to a healthy volunteer. After a 12-hour lag time, hFc gamma RI expressing
PMNs were detected in the peripheral blood. This study shows that hFc gamma
RI is an early myeloid differentiation marker that is lost during normal
final maturation. However, committed myeloid progenitor cells can be
strongly induced by G-CSF to express hFc gamma RI, ultimately resulting in
mature granulocytic cells expressing the high-affinity receptor for IgG.
This expression may have important consequences for the functional capacity
of these cells.
Volume 81,
Issue 6,
pp. 1457-1464,
03/15/1993
Copyright © 1993 by The American Society of Hematology
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