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Antibody-induced modulation and intracellular transport of CD10 and CD19
antigens in human B-cell lines: an immunofluorescence and immunoelectron
microscopy study
S Pulczynski, AM Boesen and OM Jensen
University Department of Pathology, Aarhus Amtssygehus, Denmark.
Antibody-induced antigenic modulation (AIAM) of CD10 and CD19 was studied
on NALM-6, RAJI, and JOK-1 cell lines using fluorescence microscopy (FM),
flow cytometry (FCM), and immunoelectron microscopy (IEM). Cross-linking
with monoclonal antibodies (MoAbs) induced rapid redistribution of CD10 and
CD19 on the cell surface (FM) followed by internalization involving uptake
through plasmalemmal pits, transfer through endosomal compartment
(receptor-mediated endocytosis), and, finally, delivery to lysosomes for
degradation or exocytosis and recycling (IEM). Significant quantitative
differences regarding modulation and intracellular processing were shown by
FCM and IEM. Thus, 35%, 30%, and 25% of CD10 compared with 80%, 60%, and
40% of CD19 were internalized in NALM-6, RAJI, and JOK-1 cells,
respectively. Also, the rate of intracellular transfer as well as
externalization and recycling was more pronounced in the case of CD19 than
of CD10 and in the NALM-6 and RAJI cells compared with the JOK-1 cells.
These differences may possibly reflect the functional significance of CD10
and CD19 as well as the stage of differentiation of the malignant B cells.
Although both antigens can be useful in MoAb-targeted immunotherapy, our
findings suggest that anti-CD19 MoAbs would be preferable for delivery of
cytotoxic agents to malignant B cells.
Volume 81,
Issue 6,
pp. 1549-1557,
03/15/1993
Copyright © 1993 by The American Society of Hematology
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