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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Litz, C. E. Articles by Brunning, R. D. Search for Related Content PubMed PubMed Citation Articles by Litz, C. E. Articles by Brunning, R. D. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Duplication of small segments within the major breakpoint cluster region in chronic myelogenous leukemia CE Litz, JS McClure, CM Copenhaver and RD Brunning Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis. The t(9;22) in chronic myelogenous leukemia (CML) may be reciprocal or, in a minority of cases, may result in an extensive deletion of a portion of the major breakpoint cluster region (M-bcr) of the BCR. This report provides evidence of the duplication of small segments within the M-bcr in a small group of patients with CML. Southern blots of Bgl II and Bgl II/BamHI double-digested DNA from the blood or bone marrow of 46 patients with CML were probed with a 5' 1.4-kb Taq I/HindIII M- bcr probe and a 3' 2-kb HindIII/BamHI M-bcr probe. In three patients, rearrangements were noted with both probes in Bgl II-digested DNA, but were not present in Bgl II/BamHI-digested DNA with either probe. Southern analysis of DNA samples double-digested with Bgl II and BspHI from two of these three cases showed no rearrangements with either probe; the M-bcr BspHI site is located 26 bp 3' of the BamHI site in the second intron of the M-bcr. The presence of a rearranged M-bcr with both probes in Bgl II-digested DNA and the lack of rearrangement in Bgl II/BamHI and Bgl II/BspHI double-digested DNA suggest the presence of M- bcr BamHI and BspHI sites on both 9q+ chromosome (9q+) and the Philadelphia chromosome (Ph). This implies a duplication of at least the 26-bp M-bcr BamHI/BspHI fragment in these two samples. Sequence data from one of these two cases confirmed the M-bcr breakpoints to be staggered; the Ph M-bcr breakpoint occurred 258 bp downstream from the 9q+ M-bcr breakpoint. It is concluded that a duplication of small segments within the M-bcr occurs in a small group of patients with CML, which may lead to pseudogermline patterns on Southern blot. Such a duplication may provide insight into the mechanism of some chromosomal translocations in neoplasia. Volume 81, Issue 6, pp. 1567-1572, 03/15/1993 Copyright © 1993 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. D. Putnam, V. Pennaneach, and R. D. Kolodner Saccharomyces cerevisiae as a Model System To Define the Chromosomal Instability Phenotype Mol. Cell. Biol., August 15, 2005; 25(16): 7226 - 7238. [Abstract] [Full Text] [PDF] G. Saglio, C. T. Storlazzi, E. Giugliano, C. Surace, L. Anelli, G. Rege-Cambrin, A. Zagaria, A. J. Velasco, A. Heiniger, P. Scaravaglio, et al. A 76-kb duplicon maps close to the BCR gene on chromosome 22 and the ABL gene on chromosome 9: Possible involvement in the genesis of the Philadelphia chromosome translocation PNAS, July 23, 2002; 99(15): 9882 - 9887. [Abstract] [Full Text] [PDF]

	Copyright © 1993 by American Society of Hematology         Online ISSN: 1528-0020