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Enrichment of interleukin-2-responsive natural killer progenitors in human
bone marrow
A Shibuya, H Kojima, K Shibuya, K Nagayoshi, T Nagasawa and H Nakauchi
Division of Hematology, University of Tsukuba, Ibaraki, Japan.
Natural killer (NK) cells can be cultured in interleukin-2 (IL-2)-
containing medium from selected human bone marrow (BM) cells obtained after
the elimination of mature T and NK cells. To isolate and characterize
IL-2-responsive NK progenitors in the selected BM cells, we investigated
the expression of IL-2 receptors (IL-2R) on these cells. Neither CD25
(IL-2R alpha) nor IL-2R beta antigen was observed on the selected BM cells
before culture. However, CD25+ cells without detectable levels of IL-2R
beta antigen appeared 24 hours after culture in IL-2-containing medium.
Cells were sorted from each fraction of the selected BM cells 24 hours
after culture after staining with anti-CD33, anti-CD34, and anti-CD25
monoclonal antibodies. The generation of NK cells (CD3- CD56+ cells) and NK
activity were observed only from the CD33-/CD34-/CD25+ cell fraction after
culture in IL-2-containing medium. The frequency of IL-2-responsive NK
progenitors relative to the fraction was 1/231 (95% confidence range, 1/156
to 1/289), which corresponded to the frequency relative to the total number
of selected BM cells when the frequency relative to the CD33-/CD34-/CD25+
cell- fraction was converted according to the percentage of these cells in
the total number of selected BM cells. These results indicated that IL-
2-responsive NK progenitors were enriched in the CD33-/CD34-/CD25+ cell
fraction.
Volume 81,
Issue 7,
pp. 1819-1826,
04/01/1993
Copyright © 1993 by The American Society of Hematology
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