|
|
 Previous Article | Table of Contents | Next Article 
Clonal studies of CD3- lymphoproliferative disease of granular lymphocytes
R Nash, P McSweeney, R Zambello, G Semenzato and TP Loughran
Fred Hutchinson Cancer Research Center, Seattle, WA.
The lymphoproliferative disease of granular lymphocytes (LDGL) results from
chronic proliferation of either CD3- or CD3+ large granular lymphocytes
(LGLs). Most CD3+ LGLs have been well characterized as clonal LGL
proliferations (LGL leukemia). In contrast, the clonal nature and clinical
features of patients with CD3- LDGL have not been defined. In this study,
we analyzed seven female patients with CD3- LDGL who were heterozygous at
certain X-linked gene loci. Neutrophils and CD3- granular lymphocytes were
isolated from peripheral blood. Clonal analysis was performed on genomic
DNA from these cell fractions on six patients by conventional Southern
techniques using probes to the X-linked genes, PGK and DXS255 (M27 beta).
In four patients, three of whom were already studied by Southern analyses
of genomic DNA and one in whom there were insufficient amounts of DNA,
polymerase chain reaction (PCR)-based clonal analysis was performed with
primer pairs flanking the BstXI polymorphism on the PGK gene. In six
patients, a polyclonal expansion of CD3- granular lymphocytes was
demonstrated and in one the result was indeterminate. In contrast to
patients with CD3+ LDGL (LGL leukemia), a clonal disease could not be
demonstrated with X- linked markers in patients with CD3- LDGL, suggesting
a reactive rather than a neoplastic origin of the lymphocytes in these
cases.
Volume 81,
Issue 9,
pp. 2363-2368,
05/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
X. Chen, F. Bai, L. Sokol, J. Zhou, A. Ren, J. S. Painter, J. Liu, D. A. Sallman, Y. A. Chen, J. A. Yoder, et al.
A critical role for DAP10 and DAP12 in CD8+ T cell-mediated tissue damage in large granular lymphocyte leukemia
Blood,
April 2, 2009;
113(14):
3226 - 3234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boudewijns, J. J.M. van Dongen, and A. W. Langerak
The Human Androgen Receptor X-Chromosome Inactivation Assay for Clonality Diagnostics of Natural Killer Cell Proliferations
J. Mol. Diagn.,
July 1, 2007;
9(3):
337 - 344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lima, J. Almeida, A. G. Montero, M. d. A. Teixeira, M. L. Queiros, A. H. Santos, A. Balanzategui, A. Estevinho, M. d. C. Alguero, P. Barcena, et al.
Clinicobiological, Immunophenotypic, and Molecular Characteristics of Monoclonal CD56-/+dim Chronic Natural Killer Cell Large Granular Lymphocytosis
Am. J. Pathol.,
October 1, 2004;
165(4):
1117 - 1127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. Epling-Burnette, J. S. Painter, P. Chaurasia, F. Bai, S. Wei, J. Y. Djeu, and T. P. Loughran Jr
Dysregulated NK receptor expression in patients with lymphoproliferative disease of granular lymphocytes
Blood,
May 1, 2004;
103(9):
3431 - 3439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zambello, M. Falco, M. D. Chiesa, L. Trentin, D. Carollo, R. Castriconi, G. Cannas, S. Carlomagno, A. Cabrelle, T. Lamy, et al.
Expression and function of KIR and natural cytotoxicity receptors in NK-type lymphoproliferative diseases of granular lymphocytes
Blood,
September 1, 2003;
102(5):
1797 - 1805.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-i. Mizuno, K. Akashi, K. Ohshima, H. Iwasaki, T. Miyamoto, N. Uchida, T. Shibuya, M. Harada, M. Kikuchi, and Y. Niho
Interferon-{gamma} Prevents Apoptosis in Epstein-Barr Virus-Infected Natural Killer Cell Leukemia in an Autocrine Fashion
Blood,
May 15, 1999;
93(10):
3494 - 3504.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. P. Loughran Jr, K. G. Hadlock, Q. Yang, R. Perzova, R. Zambello, G. Semenzato, S. K.H. Foung, and B. J. Poiesz
Seroreactivity to an Envelope Protein of Human T-Cell Leukemia/Lymphoma Virus in Patients With CD3- (Natural Killer) Lymphoproliferative Disease of Granular Lymphocytes
Blood,
September 1, 1997;
90(5):
1977 - 1981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zambello, M. Facco, L. Trentin, R. Sancetta, C. Tassinari, A. Perin, A. Milani, G. Pizzolo, F. Rodeghiero, C. Agostini, et al.
Interleukin-15 Triggers the Proliferation and Cytotoxicity of Granular Lymphocytes in Patients With Lymphoproliferative Disease of Granular Lymphocytes
Blood,
January 1, 1997;
89(1):
201 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
