|
|
 Previous Article | Table of Contents | Next Article 
Granulocyte-macrophage colony-stimulating factor induces sequential
activation and deactivation of binding via a low-affinity IgG Fc receptor,
hFc gamma RII, on human eosinophils
L Koenderman, SW Hermans, PJ Capel and JG van de Winkel
Department of Pulmonary Diseases, University Hospital Utrecht, The
Netherlands.
Eosinophils are important in antibody-mediated immune defense against
parasites based on interaction with Ig receptors (FcR). Of the three
classes of IgG FcR in humans, hFc gamma RI, II, and III, solely hFc gamma
RII (CD32) is expressed on freshly isolated eosinophils. Despite an
expression level similar to that found on monocytes and polymorphonuclear
granulocytes, binding activity of hFc gamma RII on eosinophils is
constitutively low. Freshly isolated eosinophils had a negligible ability
to form rosettes with IgG-sensitized erythrocytes (EA-IgG). Addition of
granulocyte-macrophage colony-stimulating factor (GM-CSF) caused an
approximately threefold increase in EA-IgG rosettes. This increase was
maximal after 35 minutes, and declined upon further incubation at 37
degrees C. Analysis of hFc gamma RII expression levels showed no
significant changes and neither was the expression of other hFc gamma R
classes induced. Blocking studies with anti-Fc gamma receptor monoclonal
antibody (MoAb) proved hFc gamma RII specificity of enhanced IgG complex
binding. These phenomena were not restricted to GM- CSF action, because the
addition of interleukin-3 or interleukin-5 similarly enhanced EA-IgG
binding. The kinetics of activation of hFc gamma RII binding activity were
paralleled by the binding of EA-C3bi to CR3 on eosinophils. In contrast to
the stable expression of hFc gamma RII during activation with GM-CSF, CR3
expression increased slowly. Ligand binding via both types of opsonin
receptors proved receptor specific. However, the kinetics of enhanced
binding via hFc gamma RII and CR3 suggested the possibility of a common
mechanism underlying the enhancement of ligand binding via hFc gamma RII
and CR3. This hypothesis was supported by the fact that binding via hFc
gamma RII proved sensitive to both high concentrations of F(ab')2 fragments
of anti-CD11b MoAb MO1 and chelation of bivalent cations with EDTA. In
conclusion, our studies indicate that cytokines can induce a transient
enhancement of hFc gamma RII binding activity. Qualitative, and not
quantitative, changes in this receptor appear to underly the modulation of
binding activity, which may be linked to changes in CR3 activity.
Volume 81,
Issue 9,
pp. 2413-2419,
05/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. E. Bakema, A. Bakker, S. de Haij, H. Honing, M. Bracke, L. Koenderman, G. Vidarsson, J. G. J. van de Winkel, and J. H. W. Leusen
Inside-Out Regulation of Fc{alpha}RI (CD89) Depends on PP2A
J. Immunol.,
September 15, 2008;
181(6):
4080 - 4088.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bracke, J.-W. J. Lammers, P. J. Coffer, and L. Koenderman
Cytokine-induced inside-out activation of Fc{alpha}R (CD89) is mediated by a single serine residue (S263) in the intracellular domain of the receptor
Blood,
June 1, 2001;
97(11):
3478 - 3483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bracke, E. van de Graaf, J.-W. J Lammers, P. J Coffer, and L. Koenderman
In vivo priming of Fc{alpha}R functioning on eosinophils of allergic asthmatics
J. Leukoc. Biol.,
November 1, 2000;
68(5):
655 - 661.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Bracke, E. Nijhuis, J.-W. J. Lammers, P. J. Coffer, and L. Koenderman
A critical role for PI 3-kinase in cytokine-induced Fcalpha -receptor activation
Blood,
March 15, 2000;
95(6):
2037 - 2043.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Cortegano, V. d. Pozo, B. Cardaba, I. Arrieta, S. Gallardo, M. Rojo, E. Aceituno, T. Takai, S. Verbeek, P. Palomino, et al.
Interaction between galectin-3 and Fc{gamma}RII induces down-regulation of IL-5 gene: implication of the promoter sequence IL-5REIII
Glycobiology,
March 1, 2000;
10(3):
237 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Giembycz and M. A. Lindsay
Pharmacology of the Eosinophil
Pharmacol. Rev.,
June 1, 1999;
51(2):
213 - 340.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bracke, P. J. Coffer, J.-W. J. Lammers, and L. Koenderman
Analysis of Signal Transduction Pathways Regulating Cytokine-Mediated Fc Receptor Activation on Human Eosinophils
J. Immunol.,
December 15, 1998;
161(12):
6768 - 6774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
