|
|
 Previous Article | Table of Contents | Next Article 
Enhancement of cathepsin G-induced platelet activation by leukocyte
elastase: consequence for the neutrophil-mediated platelet activation
P Renesto and M Chignard
Unite de Pharmacologie Cellulaire, Unite Associee Institut Pasteur/INSERM
285, Institut Pasteur, Paris, France.
We have focused our interest on the platelet-activating properties of two
polymorphonuclear neutrophil (PMN)-derived proteinases, namely elastase
(HLE) and cathepsin G (Cat.G). First of all, we observed that whereas HLE
was unable to trigger platelet activation by itself, it enhanced platelet
activation induced by Cat.G when both proteinases were added
simultaneously. It has been recently described that, upon stimulation, PMN
released Cat.G, which in turn activated surrounding platelets. Thus, we
looked for a combined effect of Cat.G and HLE during this cell-to-cell
interaction. When PMN (5 x 10(6)/mL) were stimulated by 0.5 mumol/L
N-formyl-Met-Leu-Phe, they released 237.9 +/- 49.1 nmol/L Cat.G and 381.7
+/- 28.0 nmol/L HLE. Such a concentration of purified Cat.G (240 nmol/L)
induced only a moderate platelet activation when added to a PMN-platelet
mixture. However, when Cat.G (240 nmol/L) and HLE (380 nmol/L) were added
together, the resulting platelet activation was strictly comparable to that
corresponding to the addition of N-formyl-Met-Leu-Phe (P > .05) in terms
of aggregation, dense and alpha granule secretion, and thromboxane B2
production. In fact, Elafin, a specific HLE inhibitor, when added to the
PMN-platelet cooperation system triggered by N-formyl-Met-Leu-Phe,
prevented platelet activation within the same range of concentrations as
for inhibition of HLE activity. In conclusion, we now show that not only
Cat.G, but also HLE is involved in the PMN-mediated platelet activation.
Volume 82,
Issue 1,
pp. 139-144,
07/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Blasco-Colmenares, T. M. Perl, E. Guallar, W. A. Baumgartner, J. V. Conte, D. Alejo, R. Pastor-Barriuso, A. R. Sharrett, and N. Faraday
Aspirin Plus Clopidogrel and Risk of Infection After Coronary Artery Bypass Surgery
Arch Intern Med,
April 27, 2009;
169(8):
788 - 795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, M. Handa, Y. Sato, Y. Ikeda, and S. Aiso
Membrane-proximal {alpha}/{beta} Stalk Interactions Differentially Regulate Integrin Activation
J. Biol. Chem.,
July 1, 2005;
280(26):
24775 - 24783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Beaufort, D. Leduc, J.-C. Rousselle, V. Magdolen, T. Luther, A. Namane, M. Chignard, and D. Pidard
Proteolytic Regulation of the Urokinase Receptor/CD87 on Monocytic Cells by Neutrophil Elastase and Cathepsin G
J. Immunol.,
January 1, 2004;
172(1):
540 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. El Ouriaghli, H. Fujiwara, J. J. Melenhorst, G. Sconocchia, N. Hensel, and A. J. Barrett
Neutrophil elastase enzymatically antagonizes the in vitro action of G-CSF: implications for the regulation of granulopoiesis
Blood,
March 1, 2003;
101(5):
1752 - 1758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hagiwara, T. Kikuchi, Y. Endo, Huqun, K. Usui, M. Takahashi, N. Shibata, T. Kusakabe, H. Xin, S. Hoshi, et al.
Mouse SWAM1 and SWAM2 Are Antibacterial Proteins Composed of a Single Whey Acidic Protein Motif
J. Immunol.,
February 15, 2003;
170(4):
1973 - 1979.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Valenzuela-Fernandez, T. Planchenault, F. Baleux, I. Staropoli, K. Le-Barillec, D. Leduc, T. Delaunay, F. Lazarini, J.-L. Virelizier, M. Chignard, et al.
Leukocyte Elastase Negatively Regulates Stromal Cell-derived Factor-1 (SDF-1)/CXCR4 Binding and Functions by Amino-terminal Processing of SDF-1 and CXCR4
J. Biol. Chem.,
May 3, 2002;
277(18):
15677 - 15689.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Grenier, S. Chollet-Martin, B. Crestani, C. Delarche, J. El Benna, A. Boutten, V. Andrieu, G. Durand, M.-A. Gougerot-Pocidalo, M. Aubier, et al.
Presence of a mobilizable intracellular pool of hepatocyte growth factor in human polymorphonuclear neutrophils
Blood,
April 15, 2002;
99(8):
2997 - 3004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Falanga, M. Marchetti, V. Evangelista, A. Vignoli, M. Licini, M. Balicco, S. Manarini, G. Finazzi, C. Cerletti, and T. Barbui
Polymorphonuclear leukocyte activation and hemostasis in patients with essential thrombocythemia and polycythemia vera
Blood,
December 15, 2000;
96(13):
4261 - 4266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Nemoto, S. Sugawara, H. Tada, H. Takada, H. Shimauchi, and H. Horiuchi
Cleavage of CD14 on Human Gingival Fibroblasts Cocultured with Activated Neutrophils Is Mediated by Human Leukocyte Elastase Resulting in Down-Regulation of Lipopolysaccharide-Induced IL-8 Production
J. Immunol.,
November 15, 2000;
165(10):
5807 - 5813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Le-Barillec, D. Pidard, V. Balloy, and M. Chignard
Human neutrophil cathepsin G down-regulates LPS-mediated monocyte activation through CD14 proteolysis
J. Leukoc. Biol.,
August 1, 2000;
68(2):
209 - 215.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. M. MacIvor, S. D. Shapiro, C. T.N. Pham, A. Belaaouaj, S. N. Abraham, and T. J. Ley
Normal Neutrophil Function in Cathepsin G-Deficient Mice
Blood,
December 15, 1999;
94(12):
4282 - 4293.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. von Dobschuetz, T. Hoffmann, and K. Messmer
Inhibition of Neutrophil Proteinases by Recombinant Serpin Lex032 Reduces Capillary No-Reflow in Ischemia/Reperfusion-Induced Acute Pancreatitis
J. Pharmacol. Exp. Ther.,
August 1, 1999;
290(2):
782 - 788.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Falanga, M. Marchetti, V. Evangelista, S. Manarini, E. Oldani, S. Giovanelli, M. Galbusera, C. Cerletti, and T. Barbui
Neutrophil Activation and Hemostatic Changes in Healthy Donors Receiving Granulocyte Colony-Stimulating Factor
Blood,
April 15, 1999;
93(8):
2506 - 2514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Merhi, P. Provost, P. Chauvet, J.-F. Theoret, M. L. Phillips, and J.-G. Latour
Selectin Blockade Reduces Neutrophil Interaction With Platelets at the Site of Deep Arterial Injury by Angioplasty in Pigs
Arterioscler Thromb Vasc Biol,
February 1, 1999;
19(2):
372 - 377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Brus, W. van Oeveren, A. Okken, and S. B. Oetomo
Number and Activation of Circulating Polymorphonuclear Leukocytes and Platelets Are Associated with Neonatal Respiratory Distress Syndrome Severity
Pediatrics,
May 1, 1997;
99(5):
672 - 680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Si-Tahar, D. Pidard, V. Balloy, M. Moniatte, N. Kieffer, A. Van Dorsselaer, and M. Chignard
Human Neutrophil Elastase Proteolytically Activates the Platelet Integrin alpha IIbbeta 3 through Cleavage of the Carboxyl Terminus of the alpha IIb Subunit Heavy Chain. INVOLVEMENT IN THE POTENTIATION OF PLATELET AGGREGATION
J. Biol. Chem.,
April 25, 1997;
272(17):
11636 - 11647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Renesto, M. Si-Tahar, M. Moniatte, V. Balloy, A. Van Dorsselaer, D. Pidard, and M. Chignard
Specific Inhibition of Thrombin-Induced Cell Activation by the Neutrophil Proteinases Elastase, Cathepsin G, and Proteinase 3: Evidence for Distinct Cleavage Sites Within the Aminoterminal Domain of the Thrombin Receptor
Blood,
March 15, 1997;
89(6):
1944 - 1953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
