|
|
 Previous Article | Table of Contents | Next Article 
Human recombination activating gene-1 in leukemia/lymphoma cells:
expression depends on stage of lymphoid differentiation defined by
phenotype and genotype
N Yoneda, E Tatsumi, S Kawano, Y Matsuo, J Minowada and N Yamaguchi
Department of Laboratory Medicine, Kobe University School of Medicine,
Japan.
The recombination activating gene, RAG-1, which is supposed to encode a
molecule regulating V(D)J recombination, has been isolated. In the current
study, the distribution of RAG-1 expression in human neoplastic
hematopoietic cells was compared with the phenotypic and genotypic status
of differentiation. Thirty-one hematopoietic cell lines (16 B- lineage, 9
T-lineage, 2 Hodgkin's disease, and 4 nonlymphoid cell lines) were
investigated for the expression of human RAG-1 using the
reverse-transcriptase polymerase chain reaction (RT-PCR). RAG-1 was not
expressed in nonlymphoid, Hodgkin's disease, or mature-stage lymphoid cell
lines, but was present in some acute lymphoblastic leukemia
(ALL)/lymphoblastic lymphoma (LBL) cell lines. The investigation was
extended to 45 cases of fresh ALL/LBL cells. The patterns of RAG-1
expression found in the cell lines and fresh ALL/LBL cells were similar. In
B-lineage cells, the product of RAG-1 RT-PCR was detected in CD19+ CD10-
CD20- CD5- stage (stage II, Nadler's classification) and was at the highest
level in CD19+ CD10+ CD20- CD5- stage (stage III), but was absent or
limited in CD19+ CD10+ CD20-+ CD5- (stage IV) or CD19+ CD10+ (or CD10-)
CD5+. In stage II, monoclonal gene rearrangements of only the
immunoglobulin heavy chain (IgH) were found, whereas monoclonal gene
rearrangements of both IgH and T-cell receptor (TCR)-beta chain were
frequently noted in stages III and IV. The expression of CD20 or CD5
antigen apparently correlated with the decline of RAG-1 expression. In
T-lineage cells, RAG-1 was highly expressed in CD3- CD4+ CD8+/CD3+ CD4+
CD8+ thymic stages, but was negative or only weakly expressed in the CD3-
CD4- CD8- prothymic or early thymic stage, in which the TCR-beta gene was
often germline, or the CD3+ CD4+ CD8- mature thymic stage. The relative
levels of RAG-1 mRNA give an additional delineating frame to the schemes of
lymphoid differentiation based on phenotypic and genotypic status. RAG-1 is
exhibited by cells of the thymic stage capable of synthesizing TCR or
expressing it on the cell surface. The weak or absent expression of RAG- 1
in the prothymic or early thymic stage suggests that the contribution of
RAG-1 to the gene rearrangement may differ quantitatively between
TCR-delta/TCR-gamma and TCR-beta.(ABSTRACT TRUNCATED AT 400 WORDS)
Volume 82,
Issue 1,
pp. 207-216,
07/01/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
V. Asnafi, K. Beldjord, E. Boulanger, B. Comba, P. Le Tutour, M.-H. Estienne, F. Davi, J. Landman-Parker, P. Quartier, A. Buzyn, et al.
Analysis of TCR, pTalpha , and RAG-1 in T-acute lymphoblastic leukemias improves understanding of early human T-lymphoid lineage commitment
Blood,
April 1, 2003;
101(7):
2693 - 2703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Nakamura, E. Tatsumi, S. Kawano, A. Tani, S. Kumagai, M. Nishikori, and T. Nagai
Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma of Natural Killer (NK) Lineage: Quest for Another NK-Lineage Neoplasm
Blood,
June 15, 1997;
89(12):
4665 - 4666.
[Full Text]
[PDF]
|
|
|
|
|
