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Connexin-43-type gap junctions mediate communication between bone marrow
stromal cells
K Dorshkind, L Green, A Godwin and WH Fletcher
Division of Biomedical Sciences, University of California, Riverside
92521-0121.
Several morphologic studies have suggested that gap junctions exist between
bone marrow stromal cells. This possibility was examined by analysis of
stromal cells present in the adherent layer of primary long- term lymphoid
bone marrow cultures and in additional studies using a stromal cell line.
Results showing that the fluorescent dye lucifer yellow, when microinjected
into a single stromal cell, transferred between most other contacting
stroma and that stromal cells were electronically coupled provided support
that cell-cell communication occurs between these microenvironmental
elements. Additional studies showed that transcripts for connexin (Cx) 43,
but not for Cx26 or Cx32, were present in a stromal cell line. To examine
the potential for regulated cell-cell communication between the stroma,
cells were treated with interleukin-1 (IL-1), a cytokine known to affect
stromal cell function, and the effects on dye transfer were examined. IL-1
treatment resulted in a reversible decrease in the ability of dye to
transfer between stromal cells in contact. Taken together, these studies
show that gap junctions exist between stromal cells and that their
permeability can be regulated. However, gap junction-mediated cell-cell
communication could not be shown between the stroma and developing lymphoid
cells.
Volume 82,
Issue 1,
pp. 38-45,
07/01/1993
Copyright © 1993 by The American Society of Hematology
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