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Platelet adhesion to cyanogen-bromide fragments of collagen alpha 1(I)
under flow conditions
EU Saelman, LF Horton, MJ Barnes, HR Gralnick, KM Hese, HK Nieuwenhuis, PG de Groot and JJ Sixma
Department of Haematology, University Hospital Utrecht, The Netherlands.
The aim of this investigation was to identify domains of collagen type I
that can support platelet adhesion under flow conditions. Four cyanogen
bromide (CB) fragments composing 87% of the collagen alpha 1(I)-chain were
studied under static and flow conditions. Under static conditions, bovine
and human collagen fragment alpha 1(I)CB3 induced aggregate formation,
whereas alpha 1(I)CB7 and alpha 1(I)CB8 supported adhesion of dendritic and
contact platelets. Bovine alpha 1(I)CB6 weakly supported platelet adhesion.
At shear rate 300/s, collagen fragment alpha 1(I)CB3 strongly supported
platelet adhesion, whereas lower platelet adhesion was observed to alpha
1(I)CB7 and alpha 1(I)CB8. The fragment alpha 1(I)CB6 did not support
platelet adhesion under flow conditions. Adhesion to alpha 1(I)CB3 was
completely inhibited by a low concentration (0.6 IgG microgram/mL) of
anti-GPIa monoclonal antibody (MoAb), whereas this concentration of
antibody partially inhibited adhesion to alpha 1(I)CB7 and alpha 1(I)CB8.
At higher concentrations (3 micrograms/mL) the anti-glycoprotein Ia (GPIa)
antibody completely inhibited adhesion to alpha 1(I)CB8 and further reduced
adhesion to alpha 1(I)CB7. Platelet adhesion to alpha 1(I)CB3, alpha
1(I)CB7, and alpha 1(I)CB8 was strongly inhibited by an anti-GPIb MoAb. A
MoAb against the GPIb-binding site of von Willebrand factor (vWF) strongly
inhibited platelet adhesion to alpha 1(I)CB7 and alpha 1(I)CB8, whereas
platelet adhesion to alpha 1(I)CB3 was not inhibited. We conclude that
under flow conditions alpha 1(I)CB3, alpha 1(I)CB7, and alpha 1(I)CB8
support GPIa/IIa-dependent platelet adhesion. The GPIb-vWF interaction is
important under flow conditions for adhesion to alpha 1(I)CB7 and alpha
1(I)CB8 and probably also to alpha 1(I)CB3.
Volume 82,
Issue 10,
pp. 3029-3033,
11/15/1993
Copyright © 1993 by The American Society of Hematology
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