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Activation of human platelets by immune complexes prepared with cationized
human IgG
M Schattner, M Lazzari, AS Trevani, E Malchiodi, AC Kempfer, MA Isturiz and JR Geffner
Instituto de Investigaciones Hematologicas (IIHEMA), Academia Nacional de
Medicina, Buenos Aires, Argentina.
The present study shows that the ability of soluble immune complexes (IC),
prepared with human IgG and rabbit IgG antibodies against human IgG, to
trigger platelet activation was markedly higher for IC prepared with
cationized human IgG (catIC) compared with those prepared with untreated
human IgG (cIC). CatIC induced platelet aggregation and adenosine
triphosphate release in washed platelets (WP), gel-filtered platelets
(GFP), or platelet-rich plasma (PRP) at physiologic concentrations of
platelets (3 x 10(8)/mL) and at low concentrations of catIC (1 to 30
micrograms/mL). On the contrary, under similar experimental conditions, cIC
did not induce aggregation in PRP, WP, or GFP. Low aggregation responses
were only observed using high concentrations of both WP (9 x 10(8)/mL) and
cIC (500 micrograms/mL). Interestingly, catIC were also able to induce
platelet activation under nonaggregating conditions, as evidenced by
P-selectin expression. Cationized human IgG alone did not induce platelet
aggregation in PRP but triggered either WP or GFP aggregation. However, the
concentration needed to induce these responses, was about eightfold higher
than those required for catIC. The responses induced either by catIC or
cationized human IgG were completely inhibited by treatment with heparin,
dextran sulphate, EDTA, prostaglandin E1, or IV3, a monoclonal antibody
against the receptor II for the Fc portion of IgG (Fc gamma RII). The data
presented in this study suggest that IgG charge constitutes a critical
property that conditions the ability of IC to trigger platelet activation.
Volume 82,
Issue 10,
pp. 3045-3051,
11/15/1993
Copyright © 1993 by The American Society of Hematology
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