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The novel subset of CD14+/CD16+ blood monocytes is expanded in sepsis
patients
G Fingerle, A Pforte, B Passlick, M Blumenstein, M Strobel and HW Ziegler- Heitbrock
Institute for Immunology, University of Munich, Germany.
Staining with CD14 and CD16 monoclonal antibodies will identify two
monocyte subpopulations in human blood: a major population of regular
monocytes, which strongly expresses the CD14 antigen (CD14++), and a minor
population with weak expression of CD14 and expression of the CD16 antigen
(CD14+/CD16+ cells). As shown herein, the latter cells account for 45 +/-
22 cells/microL and 9% +/- 5% of the monocytes in healthy control donors (n
= 35). In septicemia patients, the CD14+/CD16+ cells can become a major
population, with more than 50% of all monocytes in 3 of 18 patients and
with more than 500 cells in 4 of 18 cases. There was no correlation of
CD14+/CD16+ cells to any clinical parameter except for CD14+/CD16+
percentage and body temperature (P = .013). The CD14++ regular monocytes
showed a substantial decrease in CD14 antigen density in 9 of 11 patients.
Three-color immunofluorescence shows that the CD14+/CD16+ monocytes in
septicemia patients when compared with the CD14++ monocytes exhibit a
higher level of class II antigen and a lower level of CD11b and CD33
antigens, consistent with a more mature nature of the CD14+/CD16+ cells.
Levels of interleukin-6 (IL-6) were increased in septicemia patients; 3 of
5 patients with high numbers of CD14+/CD16+ cells (> 200/microL) had
high levels of IL-6 (> 250/U/mL). These data suggest that septicemia may
lead to substantial changes in blood monocyte composition and this may be
related to elevated levels of cytokines such as IL-6.
Volume 82,
Issue 10,
pp. 3170-3176,
11/15/1993
Copyright © 1993 by The American Society of Hematology
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