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Recombinant granulocyte colony-stimulating factor administration to healthy
volunteers: induction of immunophenotypically and functionally altered
neutrophils via an effect on myeloid progenitor cells
JM Kerst, M de Haas, CE van der Schoot, IC Slaper-Cortenbach, M Kleijer, AE von dem Borne and RH van Oers
Central Laboratory of the Red Cross Blood Transfusion Service, Amsterdam,
The Netherlands.
We performed a detailed kinetic study on the in vivo effect of a single
subcutaneous dose of granulocyte colony-stimulating factor (G-CSF; 300
micrograms) in four healthy individuals on the expression and function of
neutrophil Fc gamma receptors (Fc gamma R). G-CSF did not induce Fc gamma
RI (CD64) on circulating neutrophils. However, neutrophils newly formed in
response to G-CSF were Fc gamma RI positive and were able to perform
antibody-dependent cellular cytotoxicity in an Fc gamma RI- dependent way.
Fc gamma RII (CD32) expression was not changed significantly. Fc gamma RIII
(CD16, phosphatidylinositol-linked) expression, slightly increased
immediately (30 minutes) postinjection, was found to be strongly decreased
on the newly formed population. For comparison, we studied the expression
of the PI-linked proteins leukocyte alkaline phosphatase (LAP) and CD14.
Intracellular levels of LAP mirrored the biphasic expression pattern as
membrane-bound Fc gamma RIII. In contrast, CD14 expression on neutrophils
was initially constant, followed by high levels on the newly formed
neutrophils. Soluble CD14 levels were found to be elevated transiently,
whereas peak levels of soluble Fc gamma III were observed as late as 6 days
postinjection. In conclusion, we have shown that G-CSF results in an
immunophenotypically and functionally altered neutrophil population for an
important part as a result of its effect on myeloid precursor cells.
Volume 82,
Issue 11,
pp. 3265-3272,
12/01/1993
Copyright © 1993 by The American Society of Hematology
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