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Identification and characterization of a high-affinity granulocyte-
macrophage colony-stimulating factor receptor on primary rat
oligodendrocytes
GC Baldwin, EN Benveniste, GY Chung, JC Gasson and DW Golde
Department of Medicine, UCLA School of Medicine 90024-1678.
We previously showed the presence of receptors for granulocyte- macrophage
colony-stimulating factor (GM-CSF) on tumor tissues and tumor cell lines
that are derived from the neural crest. To determine whether normal neural
cells express functional GM-CSF receptors, we isolated and analyzed primary
rat brain cells, including microglia, astrocytes, and oligodendrocytes.
Scatchard analysis of equilibrium binding of 125I-GM-CSF to primary rat
oligodendrocytes showed an average of 1,110 GM-CSF binding sites per cell,
with a kd of 20 pmol/L. In six separate experiments, no specific binding
was detectable on the astrocyte population. Microglia were used in
competitive binding experiments with oligodendrocytes, and addition of
microglia did not increase the specific binding of labeled ligand to
oligodendrocytes. In dose-response assays, we measured 3H-thymidine uptake
in rat oligodendrocytes, microglia and control murine 32D cells stimulated
with various concentrations of GM-CSF. Over concentration ranges of 0.025
to 1000 pmol/L, cell proliferation and peak 3H-thymidine incorporation was
observed at approximately 30 pmol/L for both the control cells and the
oligodendrocytes. However, the microglial cells did not proliferate in
response to GM-CSF. These data indicate the presence of a functional
receptor for GM-CSF on primary rat oligodendrocytes, and suggest that
hematopoietic growth factors such as GM-CSF may play a role in nerve cell
development, function, or response to injury.
Volume 82,
Issue 11,
pp. 3279-3282,
12/01/1993
Copyright © 1993 by The American Society of Hematology
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