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Comparison of the human and mouse erythropoietin genes shows extensive
homology in the flanking regions
DL Galson, CC Tan, PJ Ratcliffe and HF Bunn
Division of Hematology-Oncology, Brigham and Women's Hospital, Boston, MA
02115.
Considerable insights into important cis regulatory elements in a gene can
be gleaned from the identification of sequence homologies among different
species. To extend and optimize the sequence comparison between human and
mouse erythropoietin (Epo) genes, we have obtained new human sequence from
5,547 to 385 bp upstream of the cap site and extended the 3' flank by 489
bp. In addition, we have obtained new sequence information on the mouse Epo
gene extending from within the 3' untranslated region (UTR) to 1,001 bp
downstream of the polyadenylation site. Analysis of these additional
sequences shows considerable homology between human and mouse Epo genes as
far as 4 kb (human) or 3 kb (mouse) upstream of the cap sites, as well as
far more homology at the 3' end than was previously realized. In addition,
both species were found to have a high frequency of short interspersed
(SINE) repetitive sequences that interrupt homologies in both the 5' flank
and within the transcription unit.
Volume 82,
Issue 11,
pp. 3321-3326,
12/01/1993
Copyright © 1993 by The American Society of Hematology
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