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Phenotypic and functional analysis of T-cell precursors in the human fetal
liver and thymus: CD7 expression in the early stages of T- and myeloid-cell
development
A Barcena, MO Muench, AH Galy, J Cupp, MG Roncarolo, JH Phillips and H Spits
Human Immunology Department, DNAX Research Institute, Palo Alto, CA
94304-1104.
It has been proposed that the CD7 molecule is the first antigen expressed
on the membrane of cells committed to the T-cell lineage during human fetal
T-cell ontogeny. To further identify the pre-T cell subpopulation that
migrates to the thymus early in ontogeny, we analyzed the phenotypic and
functional characteristics of the fetal liver populations separated on the
basis of CD7 expression. Three populations expressing different levels of
CD7 were observed: CD7bright, CD7dull, and CD7-. A CD7bright population
depleted of mature T, B, and myeloid cells (lineage negative, lin-) and
mostly composed of CD56+ CD34- natural killer cells did not mature into T
cells in a fetal thymic organ culture (FTOC) assay and was devoid of
myeloid progenitors in a clonal colony-forming cell assay. In contrast, the
CD7-/dull CD34+ lin- populations were capable of differentiating into
phenotypically mature T cells after injection into FTOC and contained early
myeloid progenitors. Here we phenotypically compared the fetal liver CD7
populations with the most immature fetal thymic subset that differentiated
in the FTOC assay, namely the triple negative (TN, CD3- CD4-CD8-)
thymocytes. Fetal TN lin- expressed high levels of CD34 marker and were
further subdivided by their expression of CD1 antigen, because CD1- TN
thymocytes express higher levels of CD34 antigen compared with CD1+ TN
cells. CD1- lin -TN thymocytes are characterized by expressing high levels
of CD2, CD7, and CD34 markers and dull levels of CD5, CD10, and CD28
molecules. We could not find fetal liver pre-T cells with a phenotype
equivalent to that of TN thymocytes. Our data show that CD7 does not
necessarily identify T-cell precursors during fetal T-cell development and
strongly support the hypothesis that the acquisition of early T-cell
markers as CD2, CD28, and CD5 molecules on the cell surface of T-cell
progenitors takes place intrathymically.
Volume 82,
Issue 11,
pp. 3401-3414,
12/01/1993
Copyright © 1993 by The American Society of Hematology
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