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Heterogeneous expression of a novel MPC-1 antigen on myeloma cells:
possible involvement of MPC-1 antigen in the adhesion of mature myeloma
cells to bone marrow stromal cells
N Huang, MM Kawano, H Harada, Y Harada, A Sakai, A Kuramoto and O Niwa
Department of Internal Medicine, Hiroshima University, Japan.
Recent immunophenotypic analysis has shown that the heterogeneous
expression of the adhesion molecule VLA-5 classifies myeloma cells into
VLA-5+ mature and VLA-5- immature subpopulations. To further clarify the
two myeloma subpopulations, we generated a monoclonal antibody, MPC- 1, by
immunizing mice with an adherent human myeloma cell line, KMS-5. The MPC-1
antibody recognized a 48-Kd surface antigen on KMS-5 but not on U-266, a
nonadherent human myeloma cell line. Specificity characterization showed
that MPC-1 antigen was expressed on mature myeloma cells, normal plasma
cells, and mature B cells, whereas pre-B cells and germinal center B cells
lacked its expression. Monocytes and a human bone marrow stromal cell line,
KM102, also expressed this antigen. Two subclones of MPC-1+ VLA-5+
(KMS-5Ad) and MPC-1-VLA-5+ (KMS- 5NAd) were separated from the KMS-5 cell
line. The KMS-5NAd adhered to KM102 more tightly than did the KMS-5NAd, and
the U-266 (MPC-1-VLA-5-) displayed almost no adherence to the KM102. The
adhesion of the KMS-5Ad was partially inhibited by the MPC-1 antibody.
These results, taken together, suggest that the MPC-1 antigen serves as a
differentiation marker for B-lineage cells, including plasma cells, and may
function as an adhesion molecule involved in the interaction of mature
myeloma cells with bone marrow stromal cells.
Volume 82,
Issue 12,
pp. 3721-3729,
12/15/1993
Copyright © 1993 by The American Society of Hematology
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