High proliferative potential colony-forming cell heterogeneity identified
using counterflow centrifugal elutriation
MC Yoder, XX Du and DA Williams
Herman B Wells Center for Pediatric Research, Howard Hughes Medical
Institute, Indiana University School of Medicine, Indianapolis 46202- 5225.
Murine high proliferative potential colony-forming cells (HPP-CFC) are
known to be heterogenous with respect to proliferative capacity and in
vitro responsiveness to hematopoietic growth factors. We have separated
HPP-CFC into several subpopulations using counterflow centrifugal
elutriation. Although HPP-CFC were identified in all of the elutriated
fractions of both C3H/HeJ and C57BI/6J bone marrow cells, the distribution
of HPP-CFC as well as of colony-forming units-granulocyte- macrophage
(CFU-GM) in each fraction differed between these two strains of inbred
mice. Six subsets of HPP-CFC were resolved that differed in growth factor
responsiveness. A low-density HPP-CFC subpopulation was isolated that was
distinct from day-12 spleen colony-forming units (CFU- S12), CFU-GM, and
bone marrow stromal cells. This unique subpopulation of HPP-CFC is rate (3%
to 9% of total HPP-CFC), appears to be lymphocyte-like in morphology, and
behaves the most primitive of the HPP-CFC subsets by requiring multiple
hematopoietic growth factors for optimal in vitro cloning. Further
characterization of this subpopulation of HPP-CFC will determine the
position of these cells in the HPP-CFC heirarchy.
Volume 82,
Issue 2,
pp. 385-391,
07/15/1993
Copyright © 1993 by The American Society of Hematology
