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Characterization and functional analysis of adult human bone marrow cell
subsets in relation to B-lymphoid development
S Pontvert-Delucq, J Breton-Gorius, C Schmitt, C Baillou, J Guichard, A Najman and FM Lemoine
Department of Hematology, Faculte de Medecine Saint Antoine, Paris, France.
To study the frontiers between pluripotent stem cells and committed
progenitors and to further define the B-cell pathway in adult bone marrow
(BM), CD34+ subpopulations and CD34- B-lineage cells were analyzed by
multiparameter flow cytometry, studied by light and electron microscopy,
and in short-term and long-term cultures (LTC). While the total CD34+ cells
represent 4.9% +/- 0.8 of BM mononuclear cells within the lymphoid-blast
window, 73.8 +/- 3.5%, 14.4 +/- 1.8% and 8.8 +/- 2.9% of them were CD34+
CD10- CD19-, CD34+ CD10+ CD19+, and CD34+ CD10+ CD19-, respectively. CD34+
CD10+ CD19+ cells represent a smal homogeneous TdT4 c micro-blast
population. Although expressing CD38 and high level of HLA-DR antigens,
like myeloid committed progenitors, they did not generate LTC, myeloid, and
T lymphoid colonies suggesting that the CD34+ CD10+ CD19+ population
represents exclusively B lymphoid committed progenitors. By contrast, all
myeloid progenitors and LTC-initiating cells were found in the CD34+ CD10-
CD19- cell fraction. This fraction appeared more heterogeneous and
contained CD38- HLA-DRlow small cells, larger blasts, and promonocyte-like
cells exhibiting small peroxidase-positive granules. Interestingly, CD10
was also present on CD34+ CD19- cells. This population mainly coexpressed
CD33 and gave rise to macrophagic colonies.
Volume 82,
Issue 2,
pp. 417-429,
07/15/1993
Copyright © 1993 by The American Society of Hematology
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