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In vivo administration of stem cell factor to mice increases the absolute
number of pluripotent hematopoietic stem cells
DM Bodine, NE Seidel, KM Zsebo and D Orlic
Clinical Hematology Branch, National Heart, Lung, and Blood Institute,
Bethesda, MD 20892.
We have examined the effects of administration of stem cell-factor (SCF) on
the number and distribution of pluripotent hematopoietic stem cells (PHSC)
in normal mice. Using the competitive repopulation assay we found that in
vivo administration of SCF increases the absolute number of PHSC per mouse
threefold. The increased numbers of PHSC are found in the peripheral blood
and spleen of the SCF-treated animals. The spleen and peripheral blood stem
cells completely repopulated the erythroid, myeloid, and lymphoid lineages
of irradiated or W/Wv hosts, similar to bone marrow PHSC. PHSC from the
peripheral blood of SCF- treated mice have a lineage marker-negative,
c-kit-positive phenotype that is indistinguishable from that of bone marrow
PHSC. The increase in the absolute number of spleen PHSC is associated with
efficient gene transfer to these cells without prior treatment with
5-fluorouracil. This is a US government work. There are no restrictions on
its use.
Volume 82,
Issue 2,
pp. 445-455,
07/15/1993
Copyright © 1993 by The American Society of Hematology
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