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Establishment and characterization of an erythropoietin-dependent subline,
UT-7/Epo, derived from human leukemia cell line, UT-7
N Komatsu, M Yamamoto, H Fujita, A Miwa, K Hatake, T Endo, H Okano, T Katsube, Y Fukumaki and S Sassa
Department of Medicine, Jichi Medical School, Tochigi, Japan.
UT-7 is a human leukemic cell line capable of growing in interleukin-3
(IL-3), granulocyte/macrophage colony-stimulating factor (GM-CSF), or
erythropoietin (Epo) (Komatsu et al, Cancer Res 51:341, 1991). To study the
effect of Epo on proliferation and differentiation of UT-7, we maintained
the UT-7 cell culture for more than 6 months in the presence of Epo. As a
result, a subline, UT-7/Epo, was established. The growth of UT-7/Epo could
be supported by Epo but not by GM-CSF or IL-3. UT- 7/Epo showed a greater
level of heme content and ratio of benzidine- positive staining cells than
did UT-7. Butyric acid promoted the synthesis of hemoglobin in UT-7/Epo,
but not UT-7. Further, the mRNA concentrations of the c-myb oncogene and
GM-CSF receptor beta-subunit were decreased substantially in UT-7/Epo
cells. These findings showed that UT-7/Epo cells had progressed further in
erythroid development than UT-7 cells, and suggested that long-term culture
in Epo had promoted this differentiation. Whereas availability of the Epo
receptor (Epo-R) for binding of Epo was reduced in UT-7/Epo cells compared
with UT-7 cells, the Epo-R showed a similar affinity for Epo. This
observation suggested that change(s) in postreceptor signaling step might
be involved in the establishment and maintenance of the UT-7/Epo phenotype.
Volume 82,
Issue 2,
pp. 456-464,
07/15/1993
Copyright © 1993 by The American Society of Hematology
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