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Platelets lacking functional CD36 (glycoprotein IV) show reduced adhesion
to collagen in flowing whole blood
M Diaz-Ricart, NN Tandon, M Carretero, A Ordinas, E Bastida and GA Jamieson
Hospital Clinic I Provincial, Facultad de Medicina, Universidad de
Barcelona, Spain.
A parallel-plate perfusion chamber has been used to evaluate the
contribution of the adhesive membrane glycoprotein CD36 (GPIV) to platelet
adhesion on type I collagen in flowing whole blood at a shear rate of 800
s-1. In one series of experiments, reconstituted normal blood (hematocrit
0.4; platelet count 1.5 x 10(5)/microL) was prepared from washed red blood
cells, plasma, and washed platelets that had been incubated with Fab
fragments of a monospecific polyclonal anti-CD36 antibody (50
micrograms/mL, 30 minutes, 37 degrees C). Percent surface coverage of
collagen-coated coverslips using reconstituted blood with antibody-blocked
platelets, as compared with paired reconstituted controls (100%), was 50%
at 2 minutes, 87% at 5 minutes, and 90% at 10 minutes. Further studies were
performed by perfusion of whole blood from a healthy donor of the
Naka-negative phenotype, whose platelets constitutively lack CD36, over
collagen-coated coverslips. In this case, percent surface coverage was 55%
of normal controls at 2 minutes, 76% of controls at 5 minutes, and 72% of
controls at 10 minutes. In both preparations, platelets lacking functional
CD36 had a statistically significant decrease (P < .005) in adhesion
after 2 minutes and 10 minutes perfusion but not at 5 minutes. These
results show that functional CD36 facilitates the rapid adhesion of
platelets to collagen and that this effect is seen at the earliest time
points of their interaction.
Volume 82,
Issue 2,
pp. 491-496,
07/15/1993
Copyright © 1993 by The American Society of Hematology
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