|
|
 Previous Article | Table of Contents | Next Article 
C-reactive protein induces human peripheral blood monocytes to synthesize
tissue factor
J Cermak, NS Key, RR Bach, J Balla, HS Jacob and GM Vercellotti
Department of Medicine, University of Minnesota, Minneapolis 55455.
The acute inflammatory response is frequently accompanied by serious
thrombotic events. We show that C-reactive protein (CRP), an acute- phase
reactant that markedly increases its serum concentration in response to
inflammatory stimuli, induced monocytes to express tissue factor (TF), a
potent procoagulant. Purified human CRP in concentrations commonly achieved
in vivo during inflammation (10 to 100 micrograms/mL) induced a 75-fold
increase in TF procoagulant activity (PCA) of human peripheral blood
mononuclear cells (PBM), with a parallel increase in TF antigen levels.
CRP-induced PCA was completely blocked by a monoclonal antibody against
human TF but not by irrelevant murine IgG. Dot blot analysis showed a
significant increase of TF mRNA after 4 hours of incubation with CRP,
followed by a peak of PCA within 6 and 8 hours. Actinomycin D and
cycloheximide blocked CRP-stimulated PCA, suggesting that de novo TF
protein synthesis was required. Endotoxin (LPS) contamination of CRP was
excluded as the mediator of TF synthesis because: (1) CRP was Limulus assay
negative; (2) induction of TF PCA by CRP was not blocked by Polymyxin B, in
contrast to LPS- induced PCA; (3) antihuman CRP IgG inhibited CRP-induced
PCA, but not LPS-induced PCA; (4) CRP was able to stimulate TF production
in LPS- pretreated PBM refractory to additional LPS stimulation; and, (5)
unlike LPS, CRP was incapable of inducing TF in human umbilical vein
endothelial cells. We suggest that CRP-mediated TF production in monocytes
may contribute to the development of disseminated intravascular coagulation
and thrombosis in inflammatory states.
Volume 82,
Issue 2,
pp. 513-520,
07/15/1993
Copyright © 1993 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. B. Grammer, W. Marz, W. Renner, B. O. Bohm, and M. M. Hoffmann
C-reactive protein genotypes associated with circulating C-reactive protein but not with angiographic coronary artery disease: the LURIC study
Eur. Heart J.,
May 21, 2008;
(2008)
ehn191v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Ortolani, A. Marzocchi, C. Marrozzini, T. Palmerini, F. Saia, N. Taglieri, F. Baldazzi, S. Silenzi, M. L. Bacchi-Reggiani, P. Guastaroba, et al.
Predictive value of high sensitivity C-reactive protein in patients with ST-elevation myocardial infarction treated with percutaneous coronary intervention
Eur. Heart J.,
May 2, 2008;
29(10):
1241 - 1249.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Sardo, S. Campo, G. Mandraffino, C. Saitta, A. Bonaiuto, M. Castaldo, M. Cinquegrani, G. Pizzimenti, and A. Saitta
Tissue Factor and Monocyte Chemoattractant Protein-1 Expression in Hypertensive Individuals with Normal or Increased Carotid Intima-Media Wall Thickness
Clin. Chem.,
May 1, 2008;
54(5):
814 - 823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Aziz, K. Berger, K. Claycombe, R. Huang, R. Patel, and G. S. Abela
Noninvasive Detection and Localization of Vulnerable Plaque and Arterial Thrombosis With Computed Tomography Angiography/Positron Emission Tomography
Circulation,
April 22, 2008;
117(16):
2061 - 2070.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wu, M. J. Stevenson, J. M. Brown, E. A. Grunz, T. L. Strawn, and W. P. Fay
C-Reactive Protein Enhances Tissue Factor Expression by Vascular Smooth Muscle Cells: Mechanisms and In Vivo Significance
Arterioscler. Thromb. Vasc. Biol.,
April 1, 2008;
28(4):
698 - 704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. M. Wouters, K. H. Groenewegen, M. A. Dentener, and J. H. J. Vernooy
Systemic Inflammation in Chronic Obstructive Pulmonary Disease: The Role of Exacerbations
Proceedings of the ATS,
December 1, 2007;
4(8):
626 - 634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Groschel, U. Ernemann, J. Larsen, M. Knauth, F. Schmidt, J. Artschwager, and A. Kastrup
Preprocedural C-Reactive Protein Levels Predict Stroke and Death in Patients Undergoing Carotid Stenting
AJNR Am. J. Neuroradiol.,
October 1, 2007;
28(9):
1743 - 1746.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Parolari, M. Camera, F. Alamanni, M. Naliato, G. L. Polvani, M. Agrifoglio, M. Brambilla, C. Biancardi, L. Mussoni, P. Biglioli, et al.
Systemic Inflammation After On-Pump and Off-Pump Coronary Bypass Surgery: A One-Month Follow-Up
Ann. Thorac. Surg.,
September 1, 2007;
84(3):
823 - 828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Ridker
C-Reactive Protein and the Prediction of Cardiovascular Events Among Those at Intermediate Risk: Moving an Inflammatory Hypothesis Toward Consensus
J. Am. Coll. Cardiol.,
May 29, 2007;
49(21):
2129 - 2138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Palmerini, A. Marzocchi, C. Marrozzini, L. B. Reggiani, C. Savini, G. Marinelli, R. Di Bartolomeo, and A. Branzi
Preoperative C-reactive protein levels predict 9-month mortality after coronary artery bypass grafting surgery for the treatment of left main coronary artery stenosis
Eur. J. Cardiothorac. Surg.,
April 1, 2007;
31(4):
685 - 690.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Bisoendial, J. J. P. Kastelein, S. L. M. Peters, J. H. M. Levels, R. Birjmohun, J. I. Rotmans, D. Hartman, J. C. M. Meijers, M. Levi, and E. S. G. Stroes
Effects of CRP infusion on endothelial function and coagulation in normocholesterolemic and hypercholesterolemic subjects
J. Lipid Res.,
April 1, 2007;
48(4):
952 - 960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. S. Jenny, N. D. Yanez, B. M. Psaty, L. H. Kuller, C. H. Hirsch, and R. P. Tracy
Inflammation Biomarkers and Near-Term Death in Older Men
Am. J. Epidemiol.,
March 15, 2007;
165(6):
684 - 695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Dasu, S. Devaraj, T. W. Du Clos, and I. Jialal
The biological effects of CRP are not attributable to endotoxin contamination: evidence from TLR4 knockdown human aortic endothelial cells
J. Lipid Res.,
March 1, 2007;
48(3):
509 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Cai, C. Song, I. Endoh, J. Goyette, W. Jessup, S. B. Freedman, H. P. McNeil, and C. L. Geczy
Serum Amyloid A Induces Monocyte Tissue Factor
J. Immunol.,
February 1, 2007;
178(3):
1852 - 1860.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Liuzzo, M. Santamaria, L. M. Biasucci, M. Narducci, V. Colafrancesco, A. Porto, S. Brugaletta, M. Pinnelli, V. Rizzello, A. Maseri, et al.
Persistent Activation of Nuclear Factor Kappa-B Signaling Pathway in Patients With Unstable Angina and Elevated Levels of C-Reactive Protein: Evidence for a Direct Proinflammatory Effect of Azide and Lipopolysaccharide-Free C-Reactive Protein on Human Monocytes Via Nuclear Factor Kappa-B Activation
J. Am. Coll. Cardiol.,
January 16, 2007;
49(2):
185 - 194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Van De Graaff, M. Dutta, P. Das, E. A. Shry, P. D. Frederick, M. Blaney, D. J. Pasta, and S. R. Steinhubl
Early Coronary Revascularization Diminishes the Risk of Ischemic Stroke With Acute Myocardial Infarction
Stroke,
October 1, 2006;
37(10):
2546 - 2551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, I. Jialal, B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, and I. Jialal
The Verdict Is Still Out
Circulation,
May 2, 2006;
113(17):
2128 - 2151.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Guven, A. Cetinkaya, M. Aral, G. Sokmen, M. A. Buyukbese, A. Guven, and N. Koksal
High-Sensitivity C-Reactive Protein in Patients with Metabolic Syndrome
Angiology,
May 1, 2006;
57(3):
295 - 302.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Montaner, I. Fernandez-Cadenas, C. A. Molina, M. Ribo, R. Huertas, A. Rosell, A. Penalba, L. Ortega, P. Chacon, and J. Alvarez-Sabin
Poststroke C-Reactive Protein Is a Powerful Prognostic Tool Among Candidates for Thrombolysis
Stroke,
May 1, 2006;
37(5):
1205 - 1210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Marso and W. R. Hiatt
Peripheral Arterial Disease in Patients With Diabetes
J. Am. Coll. Cardiol.,
March 7, 2006;
47(5):
921 - 929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Steffel, T. F. Luscher, and F. C. Tanner
Tissue Factor in Cardiovascular Diseases: Molecular Mechanisms and Clinical Implications
Circulation,
February 7, 2006;
113(5):
722 - 731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Mangiafico, F. Sarnataro, M. Mangiafico, and C. E. Fiore
Impaired cognitive performance in asymptomatic peripheral arterial disease: relation to C-reactive protein and D-dimer levels
Age Ageing,
January 1, 2006;
35(1):
60 - 65.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Pitiphat, M. W. Gillman, K. J. Joshipura, P. L. Williams, C. W. Douglass, and J. W. Rich-Edwards
Plasma C-Reactive Protein in Early Pregnancy and Preterm Delivery
Am. J. Epidemiol.,
December 1, 2005;
162(11):
1108 - 1113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Ray and C. P. Cannon
The Potential Relevance of the Multiple Lipid-Independent (Pleiotropic) Effects of Statins in the Management of Acute Coronary Syndromes
J. Am. Coll. Cardiol.,
October 18, 2005;
46(8):
1425 - 1433.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Cirillo, P. Golino, P. Calabro, G. Cali, M. Ragni, S. De Rosa, G. Cimmino, M. Pacileo, R. De Palma, L. Forte, et al.
C-reactive protein induces tissue factor expression and promotes smooth muscle and endothelial cell proliferation
Cardiovasc Res,
October 1, 2005;
68(1):
47 - 55.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Singh, S. Devaraj, and I. Jialal
C-Reactive Protein Decreases Tissue Plasminogen Activator Activity in Human Aortic Endothelial Cells: Evidence that C-Reactive Protein Is a Procoagulant
Arterioscler. Thromb. Vasc. Biol.,
October 1, 2005;
25(10):
2216 - 2221.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Koh, S. H. Han, M.-S. Shin, J. Y. Ahn, Y. Lee, and E. K. Shin
Significant differential effects of lower doses of hormone therapy or tibolone on markers of cardiovascular disease in post-menopausal women: a randomized, double-blind, crossover study
Eur. Heart J.,
July 2, 2005;
26(14):
1362 - 1368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Bisoendial, J. J.P. Kastelein, J. H.M. Levels, J. J. Zwaginga, B. van den Bogaard, P. H. Reitsma, J. C.M. Meijers, D. Hartman, M. Levi, and E. S.G. Stroes
Activation of Inflammation and Coagulation After Infusion of C-Reactive Protein in Humans
Circ. Res.,
April 15, 2005;
96(7):
714 - 716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. MacCallum
Markers of Hemostasis and Systemic Inflammation in Heart Disease and Atherosclerosis in Smokers
Proceedings of the ATS,
April 1, 2005;
2(1):
34 - 43.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. F. Tapson
The Role of Smoking in Coagulation and Thromboembolism in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS,
April 1, 2005;
2(1):
71 - 77.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y Sato, K Hatakeyama, A Yamashita, K Marutsuka, A Sumiyoshi, and Y Asada
Proportion of fibrin and platelets differs in thrombi on ruptured and eroded coronary atherosclerotic plaques in humans
Heart,
April 1, 2005;
91(4):
526 - 530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. R. Clapp, G. M. Hirschfield, C. Storry, J. R. Gallimore, R. P. Stidwill, M. Singer, J. E. Deanfield, R. J. MacAllister, M. B. Pepys, P. Vallance, et al.
Inflammation and Endothelial Function: Direct Vascular Effects of Human C-Reactive Protein on Nitric Oxide Bioavailability
Circulation,
March 29, 2005;
111(12):
1530 - 1536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Chait, C. Y. Han, J. F. Oram, and J. W. Heinecke
Thematic review series: The Immune System and Atherogenesis. Lipoprotein-associated inflammatory proteins: markers or mediators of cardiovascular disease?
J. Lipid Res.,
March 1, 2005;
46(3):
389 - 403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nissen, E. M. Tuzcu, P. Schoenhagen, T. Crowe, W. J. Sasiela, J. Tsai, J. Orazem, R. D. Magorien, C. O'Shaughnessy, P. Ganz, et al.
Statin Therapy, LDL Cholesterol, C-Reactive Protein, and Coronary Artery Disease
N. Engl. J. Med.,
January 6, 2005;
352(1):
29 - 38.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-B. Leu, C.-P. Lin, W.-T. Lin, T.-C. Wu, and J.-W. Chen
Risk Stratification and Prognostic Implication of Plasma Biomarkers in Nondiabetic Patients With Stable Coronary Artery Disease: The Role of High-Sensitivity C-Reactive Protein
Chest,
October 1, 2004;
126(4):
1032 - 1039.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Koh, M.-S. Shin, I. Sakuma, J. Y. Ahn, D. K. Jin, H. S. Kim, D. S. Kim, S. H. Han, W.-J. Chung, and E. K. Shin
Effects of Conventional or Lower Doses of Hormone Replacement Therapy in Postmenopausal Women
Arterioscler. Thromb. Vasc. Biol.,
August 1, 2004;
24(8):
1516 - 1521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Napoleone, A. Di Santo, G. Peri, A. Mantovani, G. de Gaetano, M. B. Donati, and R. Lorenzet
The long pentraxin PTX3 up-regulates tissue factor in activated monocytes: another link between inflammation and clotting activation
J. Leukoc. Biol.,
July 1, 2004;
76(1):
203 - 209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. K. Koh and I. Sakuma
Should Progestins Be Blamed for the Failure of Hormone Replacement Therapy to Reduce Cardiovascular Events in Randomized Controlled Trials?
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2004;
24(7):
1171 - 1179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kluft
Identifying patients at risk of coronary vascular disease: the potential role of inflammatory markers
Eur. Heart J. Suppl.,
July 1, 2004;
6(suppl_C):
C21 - C27.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Jialal, S. Devaraj, and S. K. Venugopal
C-Reactive Protein: Risk Marker or Mediator in Atherothrombosis?
Hypertension,
July 1, 2004;
44(1):
6 - 11.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. G. Conway, P. Buggins, E. Hughes, and G. Y. H. Lip
Relationship of interleukin-6 and C-Reactive protein to the prothrombotic state in chronic atrial fibrillation
J. Am. Coll. Cardiol.,
June 2, 2004;
43(11):
2075 - 2082.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Paffen, H. L. Vos, and R. M. Bertina
C-Reactive Protein Does Not Directly Induce Tissue Factor in Human Monocytes
Arterioscler. Thromb. Vasc. Biol.,
May 1, 2004;
24(5):
975 - 981.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. J Nicklas, W. Ambrosius, S. P Messier, G. D Miller, B. W. Penninx, R. F Loeser, S. Palla, E. Bleecker, and M. Pahor
Diet-induced weight loss, exercise, and chronic inflammation in older, obese adults: a randomized controlled clinical trial
Am. J. Clinical Nutrition,
April 1, 2004;
79(4):
544 - 551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
